Render Target: STATIC
Render Timestamp: 2024-12-26T11:17:10.982Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:56:12.494
Product last modified at: 2024-12-17T18:58:55.166Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Endophilin-1 (E1E6Q) Rabbit mAb #65169

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 41
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Endophilin-1 (E1E6Q) Rabbit mAb recognizes endogenous levels of total endophilin-1 protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly268 of human endophilin-1 protein.

    Background

    Endophilin proteins are part of a large family of Bin/Amphiphysin/Rvs (BAR) domain proteins that are involved in cell membrane remodeling (1). The endophilins are encoded by five genes, which produce endophilin A 1-3 and B 1-2 (2). Endophilins are involved in many cellular mechanisms, such as synaptic vesicle recycling, receptor trafficking, and membrane remodeling processes (2). Research studies indicate that endophilin 1 (endophilin A1, SH3GL2) can induce different membrane shapes (3) and participate in the morphogenesis of dendritic spines (4). Endophilin 1 is also involved in regulating blood brain barrier permeability via the EGFR-JNK pathway (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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