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XML generation date: 2024-11-22 18:01:12.507
Product last modified at: 2024-12-19T13:00:17.826Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a
  1. Products /
  2. Primary Antibodies /
  3. ER and Golgi-Associated Marker Proteins Antibody Sampler Kit

ER and Golgi-Associated Marker Proteins Antibody Sampler Kit #12718

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The ER and Golgi-Associated Marker Proteins Antibody Sampler Kit contains reagents to examine proteins that help regulate protein folding and vesicle trafficking. This kit includes enough antibody to perform two western blot experiments with each primary antibody.

    Specificity / Sensitivity

    Each antibody will detect endogenous total levels of their target protein.

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human calnexin, the sequence of human PDI, the residues surrounding Met279 of human ERp72 protein, the residues surrounding Gly147 of human RCAS1 protein, and the residues surrounding Tyr140 of mouse syntaxin 6 protein.

    Background

    Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation, and folding. The ER contains a pool of molecular chaperones to help proteins fold properly. Calnexin is a calcium-binding, ER membrane protein that ensures proper protein folding by retaining newly synthesized glycoproteins within the ER l (1-3). The specificity of calnexin for a subset of glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein (3). Many secretory proteins require the formation of intra- or inter-molecular disulfide bonds to reach their native conformation (4). Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of disulfide bonds during oxidative protein folding (5). The ER-protein Ero1 oxidizes PDI through disulfide exchange, which is followed by PDI-catalyzed disulfide bond formation in folding proteins (6). The ER stress protein 72 (ERp72) contains three thioredoxin homology domains and plays a role in the formation and isomerization of disulfide bonds (7,8).

    The tumor-associated antigen RCAS1 negatively regulates cytotoxic T lymphocyte (CTL) cytolytic activity, which impacts vesicle formation, secretion, and protein glycosylation (9-12). Overexpression of RCAS1 impairs CTL cytolytic function by negatively regulating trans-Golgi to secretory lysosome protein trafficking, leading to a delay in ER to Golgi vesicle transport and mislocalization of ER quality control and glycosylation proteins. As a result, RCAS1 induces deposition of tumor-associated glycan antigens on the cell surface, which may contribute to tumor pathogenesis through the mediation of adhesion, invasion, and metastasis (13,14). Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (15,16) that is localized to the trans-Golgi and within endosomes. It regulates membrane trafficking by partnering with a variety of other SNARE proteins (17-19) and is involved in the regulation of GLUT4 trafficking, neutrophil exocytosis, and granule secretion (20-22).
    1. Rajagopalan, S. et al. (1994) Science 263, 387-90.
    2. Bergeron, J.J. et al. (1994) Trends Biochem Sci 19, 124-8.
    3. Williams, D.B. (2006) J Cell Sci 119, 615-23.
    4. Huppa, J.B. and Ploegh, H.L. (1998) Cell 92, 145-8.
    5. Ellgaard, L. and Ruddock, L.W. (2005) EMBO Rep 6, 28-32.
    6. Tu, B.P. and Weissman, J.S. (2004) J Cell Biol 164, 341-6.
    7. Mazzarella, RA et al. (1990) J Biol Chem 265(2), 1094-101.
    8. Satoh, M et al. (2005) Cell Stress Chaperones 10(4), 278-84
    9. Rüder, C. et al. (2009) J Clin Invest 119, 2184-203.
    10. Reimer, T.A. et al. (2005) BMC Cancer 5, 47.
    11. Wolf, J. et al. (2010) FASEB J 24, 4000-19.
    12. Engelsberg, A. et al. (2003) J Biol Chem 278, 22998-3007.
    13. Bock, J.B. et al. (2001) Nature 409, 839-41.
    14. Bock, J.B. et al. (1996) J Biol Chem 271, 17961-5.
    15. Wendler, F. and Tooze, S. (2001) Traffic 2, 606-11.
    16. Bock, J.B. et al. (1997) Mol Biol Cell 8, 1261-71.
    17. Mallard, F. et al. (2002) J Cell Biol 156, 653-64.
    18. Perera, H.K. et al. (2003) Mol Biol Cell 14, 2946-58.
    19. Shewan, A.M. et al. (2003) Mol Biol Cell 14, 973-86.
    20. Martín-Martín, B. et al. (2000) Blood 96, 2574-83.
    21. Wendler, F. et al. (2001) Mol Biol Cell 12, 1699-709.
    22. Kuliawat, R. et al. (2004) Mol Biol Cell 15, 1690-701.

    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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