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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Notch2 (8A1) Rabbit mAb #2420

Filter:
  • WB
  • IP
Western Blotting Image 1: Notch2 (8A1) Rabbit mAb
Western blot analysis of extracts from SNB19 and 293 cells, untreated or stably expressing human Notch2, using Notch2 (8A1) Rabbit mAb. The full-length (FL) and the extracellular domain (EC) of Notch2 are indicated.

To Purchase # 2420

Cat. # Size Qty. Price Ships
2420T 20 µl
$152 Jan 9
2420S 100 µl
$336 Jan 9

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 230, 300
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Notch2 (8A1) Rabbit mAb detects endogenous levels of total Notch2 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein consisting of the heterodimerization domain of human Notch2.

Background

Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate (1). Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer composed of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD) (2). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes (3,4).

Notch2 is a member of Notch family and mutation in Notch2 is associated with Alagille syndrome (5).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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