Render Target: STATIC
Render Timestamp: 2024-12-20T11:43:39.153Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:24:12.754
Product last modified at: 2024-08-20T12:15:35.446Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-PSD95 (Tyr236/Tyr240) Antibody #3919

Filter:
  • WB

    Supporting Data

    REACTIVITY R
    SENSITIVITY Endogenous
    MW (kDa) 95
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-PSD95 (Tyr236/Tyr240) Antibody detects endogenous levels of PSD95 protein only when phosphorylated at Tyr236 or Tyr240.

    Species Reactivity:

    Rat

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Human, Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr236 and Tyr240 of human PSD95. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Postsynaptic Density protein 95 (PSD95) is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. These family members consist of an amino-terminal variable segment followed by three PDZ domains, an SH3 domain, and an inactive guanylate kinase (GK) domain. PSD95 is a scaffolding protein involved in the assembly and function of the postsynaptic density complex (1-2). PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs) (3). It is implicated in experience-dependent plasticity and plays an indispensable role in learning (4). Mutations in PSD95 are associated with autism (5).

    Phospho-PSD95 (Tyr236/Tyr240) Antibody is directed against previously unpublished PSD95 tyrosine phosphorylation sites at Tyr236 and Tyr240 that were identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of PSD95 at Tyr236 and Tyr240 was observed in extracts isolated from ischemic rat brain. The sites were independently found in a large-scale identification of tyrosine phosphorylation sites from murine brain (6).
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