PU.1 Antibody #2266
- WB
- IP
- IHC
- IF
- F
- ChIP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:100 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
| Chromatin IP | 1:25 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Background
- Lloberas, J. et al. (1999) Immunol Today 20, 184-9.
- Klemsz, M.J. et al. (1990) Cell 61, 113-24.
- Dahl, R. and Simon, M.C. (2003) Blood Cells Mol Dis 31, 229-33.
- DeKoter, R.P. and Singh, H. (2000) Science 288, 1439-41.
- Pongubala, J.M. et al. (1993) Science 259, 1622-5.
- Wang, J.M. et al. (2003) Mol Cell Biol 23, 1896-909.
- Zhang, P. et al. (1999) Proc Natl Acad Sci U S A 96, 8705-10.
- Moreau-Gachelin, F. et al. (1988) Nature 331, 277-80.
Limited Uses
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