Render Target: STATIC
Render Timestamp: 2024-12-20T11:42:58.069Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-04-24 09:09:29.624
Product last modified at: 2024-12-11T21:15:13.786Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

SSEA4 (MC813) Mouse mAb #4755

Filter:
  • IF
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) N/A
    Source/Isotype Mouse IgG3
    Application Key:
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:400
    Flow Cytometry (Fixed/Permeabilized) 1:200 - 1:800
    Flow Cytometry (Live) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    SSEA4 (MC813) Mouse mAb detects endogenous levels of SSEA4 antigen.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by injecting animals with human embryonal carcinoma 2102Ep cl.2A6 cells.

    Background

    SSEA4 (stage-specific embryonic antigen 4) is a glycolipid carbohydrate epitope expressed on the surface of human teratocarcinoma stem cells, human embryonic germ cells, and human embryonic stem cells (1). Expression of human SSEA4 decreases following differentiation of human embryonal carcinoma cells. Expression of the SSEA4 antigen is absent in murine pluripotent cells, but increases following differentiation (1,2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.