Render Target: STATIC
Render Timestamp: 2024-12-26T11:45:24.820Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-08-01 15:26:38.781
Product last modified at: 2024-11-27T19:30:09.779Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

ST14/Matriptase Antibody #19271

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 80, 95
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ST14/Matriptase Antibody recognizes endogenous levels of total ST14/Matriptase protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn280 of human ST14/Matriptase protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Suppressor of tumorigenicity 14 protein (ST14), also known as matriptase, is a type II transmembrane protease (1,2). It is highly expressed in epithelia cells and important for the differentiation and homeostasis of epithelium (3). ST14 is synthesized as a single-chain precursor, and cleaved first at Gly149 in endoplasmic reticulum or Golgi. The resulting N-terminal and C-terminal fragments are non-covalently associated, and interact with hepatocyte growth factor activator inhibitor 1 (HAI-1), which facilitates the transportation of ST14 to the plasma membrane. Cell surface ST14 can be activated by autocleavage at Arg614 though an incompletely understood mechanism. HAI-1 inhibits activated ST14, and HAI-1 and ST14 complex can be shed from cell surface (4,5). In different context, ST14 has been reported either as a tumor suppressor or as a tumor promoter (6-8)
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