Name: Troponin I Antibody
Price: 302.00 USD
Availability: InStock

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WB

Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	28
SOURCE	Rabbit

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Troponin I Antibody detects endogenous levels of total troponin I, both skeletal muscle and cardiac isoforms.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with two synthetic peptides corresponding to the sequence of human troponin I. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Troponin, working in conjunction with tropomyosin, functions as a molecular switch that regulates muscle contraction in response to changes in the intracellular Ca²⁺ concentration. Troponin consists of three subunits: the Ca²⁺-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca²⁺ sensitivity by reducing the Ca²⁺ binding affinity of TnC (1-3).

Database Links

UniProt ID: P19429

Entrez-Gene Id: 7137

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Troponin I Antibody #4002