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Anti-Human IgG, Fc gamma Fragment Specific, HRP-Linked Antibody

Anti-Human IgG, Fc gamma Fragment Specific, HRP-Linked Antibody #32935

Supporting Data

REACTIVITY	H
SENSITIVITY
MW (kDa)
Source/Isotype	Goat

Species Cross-Reactivity Key:

H-Human

Product Information

Product Description

Affinity purified goat Anti-Human IgG, Fc gamma Fragment Specific antibody is conjugated to horseradish peroxidase (HRP). This product has been optimized for use as a secondary antibody in Western blotting and ELISA applications.

Product Usage Information

Reconstitute with 1.0 ml of dH₂0 to create a 0.8 mg/ml working solution. Centrifuge if the solution is not clear. It is recommended to prepare the working solution on day of use.

Recommended Antibody Dilutions:

ELISA (chromogenic substrates): 1:5K-1:100K

Western Blotting (chromogenic substrates): 1:5K - 1:100K

Western Blotting (ECL substrates): 1:10K - 1:200K

Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.

Storage

Supplied lyophilized with a formulation of 0.01M Sodium Phosphate (pH 7.6), 0.25M NaCl, and 15 mg/ml bovine serum albumin (BSA). Store lyophilized at 4ºC. In lyophilized form, the product is stable for 24 months. Once in solution, store at -80ºC or add glycerol (ACS grade or better) to a final concentration of 50% and store at -20ºC. Solutions should be used within 12 months. Aliquot to avoid multiple freeze/thaw cycles.

Specificity / Sensitivity

Anti-Human IgG, Fc gamma Fragment Specific, HRP-Linked Antibody detects the Fc gamma portion of human IgG but does not cross-react with the F(ab) portion of human IgG. It does not detect human IgM or IgA, or detect other non-immunoglobulin serum proteins. This antibody has minimal cross-reaction with bovine, horse, and mouse serum proteins. It may cross-react with immunoglobulins from other species.

Species Reactivity:

Human

Source / Purification

Anti-Human IgG, Fc gamma Fragment Specific, HRP-Linked Antibody is produced by immunizing goats with human IgG. The Anti-Human IgG, Fc gamma Fragment is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Background

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.

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