Revision 3
#9931
Store at -20C
Phospho-Chk1/2 Antibody Sampler Kit
1 Kit
(9 x 20 microliters)
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
---|---|---|---|---|
Phospho-Chk1 (Ser317) (D12H3) XP® Rabbit mAb | 12302 | 20 µl | 56 kDa | Rabbit IgG |
Phospho-Chk1 (Ser345) (133D3) Rabbit mAb | 2348 | 20 µl | 56 kDa | Rabbit IgG |
Phospho-Chk1 (Ser296) Antibody | 2349 | 20 µl | 56 kDa | Rabbit |
Chk1 (2G1D5) Mouse mAb | 2360 | 20 µl | 56 kDa | Mouse IgG1 |
Chk2 (D9C6) Rabbit mAb | 6334 | 20 µl | 62 kDa | Rabbit IgG |
Phospho-Chk2 (Ser19) Antibody | 2666 | 20 µl | 62 kDa | Rabbit |
Phospho-Chk2 (Ser33/35) Antibody | 2665 | 20 µl | 62 kDa | Rabbit |
Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb | 2197 | 20 µl | 62 kDa | Rabbit IgG |
Phospho-Chk2 (Ser516) Antibody | 2669 | 20 µl | 62 kDa | Rabbit |
Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
Storage
Background
Chk2 is the mammalian homologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (5-7). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (8). Indeed, after DNA damage by ionizing radiation (IR), UV irradiation and DNA replication blocked by hydroxyurea, Thr68 and other sites in this region become phosphorylated by ATM/ATR (9-11). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain (12).
Background References
- Liu, Q. et al. (2000) Genes Dev 14, 1448-59.
- Zhao, H. and Piwnica-Worms, H. (2001) Mol Cell Biol 21, 4129-39.
- Jiang, K. et al. (2003) J Biol Chem 278, 25207-17.
- Martin, S.A. and Ouchi, T. (2008) Mol Cancer Ther 7, 2509-16.
- Chen, M.S. et al. (2003) Mol Cell Biol 23, 7488-97.
- Zeng, Y. et al. (1998) Nature 395, 507-10.
- Löffler, H. et al. (2006) Cell Cycle 5, 2543-7.
- Zachos, G. et al. (2007) Dev Cell 12, 247-60.
- Garber, K. (2005) J Natl Cancer Inst 97, 1026-8.
- Allen, J.B. et al. (1994) Genes Dev 8, 2401-15.
- Weinert, T.A. et al. (1994) Genes Dev 8, 652-65.
- Murakami, H. and Okayama, H. (1995) Nature 374, 817-9.
- Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86.
- Matsuoka, S. et al. (2000) Proc Natl Acad Sci U S A 97, 10389-94.
- Melchionna, R. et al. (2000) Nat Cell Biol 2, 762-5.
- Ahn, J.Y. et al. (2000) Cancer Res 60, 5934-6.
Trademarks and Patents
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Limited Uses
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