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CRL4/CRBN Targeted Protein Degradation Complex Antibody Sampler Kit
1 Kit
(6 x 20 microliters)
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
---|---|---|---|---|
CRBN (D8H3S) Rabbit mAb | 71810 | 20 µl | 55 kDa | Rabbit IgG |
CUL4A Antibody | 2699 | 20 µl | 80, 82 kDa | Rabbit |
DDB-1 (D4C8) Rabbit mAb | 6998 | 20 µl | 127 kDa | Rabbit IgG |
RBX1 (D3J5I) Rabbit mAb | 11922 | 20 µl | 13 kDa | Rabbit IgG |
NEDD8 (19E3) Rabbit mAb | 2754 | 20 µl | 9 kDa | Rabbit IgG |
Ubiquitin (E4I2J) Rabbit mAb | 43124 | 20 µl | Rabbit IgG | |
Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The CRL4/CRBN Targeted Protein Degradation Complex Antibody Sampler Kit provides an economical means of detecting the individual components of a CRL4/CRBN E3 ubiquitin ligase complex, including free and conjugated forms of both NEDD8 and ubiquitin. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
Targeted protein degradation is an experimental method of drug-based protein targeting that leverages endogenous proteolysis machinery, such as the the ubiquitin proteasome system (UPS), to selectively degrade proteins of interest (POIs). It is being actively explored as a therapeutic strategy to target and degrade POIs that contribute to disease progression (1). This approach differs from traditional small-molecule therapeutics that seek to suppress disease proteins (e.g., kinases) by sterically blocking catalytic domains. Proteolysis-targeting chimeras (PROTACs) and "molecular glue" degraders are the two primary degrader modalities used in UPS-mediated targeted protein degradation. PROTACs are bivalent, chemically-linked ligands that induce proximity between a POI and an E3 ubiquitin ligase, resulting in ubiquitination of the target protein, and its subsequent degradation by the UPS (2,3). Molecular glues are molecules that chemically generate novel interaction surfaces between two proteins, which can used to induce proximity between a POI and an E3 ligase. Cereblon (CRBN) is the substrate-recognition component of a Cullin-RING-ubiquitin (E3) ligase complex (CRL4/CRBN) that was among the first to be recognized for its therapeutic potential via targeted protein degradation (4). The CRL4/CRBN complex is comprised of CRBN, DDB-1, RBX1, and the scaffold protein CUL4A; its ligase activity is dynamically regulated via the covalent modification (neddylation) of CUL4A by NEDD8 (5). In unrelated mechanistic studies of multiple myeloma drugs, it was revealed that phthalimides (e.g., thalidomide, lenalidomide) promoted CRBN-dependent recruitment, ubiquitination, and proteasomal degradation of the transcription factors Ikaros (IKZF1) and Aiolos (IKZF3) (6). The discovery that phthalimides were functioning as molecular glue degraders that could selective degrade what were previously considered "undruggable" targets, led to a rapid acceleration and expansion of research into targeted protein degradation, with the promise of novel therapies for diseases deemed largely intractable using conventional small-molecule therapies (7-9).
Background References
- Bond, M.J. and Crews, C.M. (2021) RSC Chem Biol 2, 725-742.
- Sakamoto, K.M. et al. (2001) Proc Natl Acad Sci U S A 98, 8554-9.
- Sakamoto, K.M. et al. (2003) Mol Cell Proteomics 2, 1350-8.
- Krönke, J. et al. (2014) Science 343, 301-5.
- Hofmann, H. et al. (2013) J Virol 87, 11741-50.
- Lu, G. et al. (2014) Science 343, 305-9.
- Shirasaki, R. et al. (2021) Cell Rep 34, 108532.
- Alabi, S.B. and Crews, C.M. (2021) J Biol Chem 296, 100647.
- Alabi, S. et al. (2021) Nat Commun 12, 920.
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