CRL4/CRBN Targeted Protein Degradation Complex Antibody Sampler Kit #72032
Product Information
Kit Usage Information
Protocols
- 2699: Western Blotting, Immunoprecipitation (Agarose)
- 2754: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin)
- 6998: Western Blotting
- 7074: Western Blotting
- 11922: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin)
- 43124: Western Blotting
- 71810: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin)
Product Description
Specificity / Sensitivity
Each antibody in the CRL4/CRBN Targeted Protein Degradation Complex Antibody Sampler Kit detects endogenous levels of its target protein. NEDD8 (19E3) Rabbit mAb detects endogenous levels of both free and conjugated NEDD8 protein. The antibody does not cross-react with other ubiquitin family members, including ubiquitin, SUMO-1, SUMO-2, SUMO-3, and ISG15. Ubiquitin (E4I2J) Rabbit mAb recognizes endogenous levels of free ubiquitin and polyubiquitinated proteins. This antibody is able to detect free ubiquitin, linear polyubiquitin (M1-linked), and homotypic polyubiquitin chains consisting of K6, K11, K27, K29, K33, K48, and K63 linkages.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro44 of human CRBN protein, Gly832 of human DDB-1 protein, Gly35 of human ubiquitin protein, the amino terminus of human NEDD8 protein, and the carboxy terminus of human RBX1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser12 of human CUL4A. Antibodies are purified by peptide affinity chromatography.
Background
Targeted protein degradation is an experimental method of drug-based protein targeting that leverages endogenous proteolysis machinery, such as the the ubiquitin proteasome system (UPS), to selectively degrade proteins of interest (POIs). It is being actively explored as a therapeutic strategy to target and degrade POIs that contribute to disease progression (1). This approach differs from traditional small-molecule therapeutics that seek to suppress disease proteins (e.g., kinases) by sterically blocking catalytic domains. Proteolysis-targeting chimeras (PROTACs) and "molecular glue" degraders are the two primary degrader modalities used in UPS-mediated targeted protein degradation. PROTACs are bivalent, chemically-linked ligands that induce proximity between a POI and an E3 ubiquitin ligase, resulting in ubiquitination of the target protein, and its subsequent degradation by the UPS (2,3). Molecular glues are molecules that chemically generate novel interaction surfaces between two proteins, which can used to induce proximity between a POI and an E3 ligase. Cereblon (CRBN) is the substrate-recognition component of a Cullin-RING-ubiquitin (E3) ligase complex (CRL4/CRBN) that was among the first to be recognized for its therapeutic potential via targeted protein degradation (4). The CRL4/CRBN complex is comprised of CRBN, DDB-1, RBX1, and the scaffold protein CUL4A; its ligase activity is dynamically regulated via the covalent modification (neddylation) of CUL4A by NEDD8 (5). In unrelated mechanistic studies of multiple myeloma drugs, it was revealed that phthalimides (e.g., thalidomide, lenalidomide) promoted CRBN-dependent recruitment, ubiquitination, and proteasomal degradation of the transcription factors Ikaros (IKZF1) and Aiolos (IKZF3) (6). The discovery that phthalimides were functioning as molecular glue degraders that could selective degrade what were previously considered "undruggable" targets, led to a rapid acceleration and expansion of research into targeted protein degradation, with the promise of novel therapies for diseases deemed largely intractable using conventional small-molecule therapies (7-9).
- Bond, M.J. and Crews, C.M. (2021) RSC Chem Biol 2, 725-742.
- Sakamoto, K.M. et al. (2001) Proc Natl Acad Sci U S A 98, 8554-9.
- Sakamoto, K.M. et al. (2003) Mol Cell Proteomics 2, 1350-8.
- Krönke, J. et al. (2014) Science 343, 301-5.
- Hofmann, H. et al. (2013) J Virol 87, 11741-50.
- Lu, G. et al. (2014) Science 343, 305-9.
- Shirasaki, R. et al. (2021) Cell Rep 34, 108532.
- Alabi, S.B. and Crews, C.M. (2021) J Biol Chem 296, 100647.
- Alabi, S. et al. (2021) Nat Commun 12, 920.
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