Revision 1

#85679

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
13

Source/Isotype:
Rabbit IgG

UniProt ID:
#O43927

Entrez-Gene Id:
10563

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:300 - 1:1200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

CXCL13 (F6A4R) Rabbit mAb recognizes endogenous levels of total CXCL13 protein. This antibody detects 58 and 75 kDa bands of unknown origin in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CXCL13 protein.

Background

C-X-C motif chemokine ligand 13 (CXCL13), also known as B cell-attracting chemokine 1 (BCA-1), is a chemokine expressed primarily by follicular dendritic cells of secondary lymphoid organs such as lymph nodes (LNs), Peyer’s patches, and spleen (1,2). CXCL13 is the ligand for CXCR5, and upon binding, the CXCL13/CXCR5 axis is required for B cell activation, migration, and homeostasis (1-4). Dysregulated CXCL13 expression has been associated with autoimmunity, cancer, and infectious diseases, making it a potential therapeutic target (5).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

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Western blot analysis of extracts from various human cell lines and tissue using CXCL13 (F6A4R) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of CXCL13 protein in HeLa cells is consistent with the predicted expression pattern.
Western Blotting Image 1: CXCL13 (F6A4R) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human CXCL13 protein (hCXCL13; +), using CXCL13 (F6A4R) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: CXCL13 (F6A4R) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using CXCL13 (F6A4R) Rabbit mAb.
Immunohistochemistry Image 1: CXCL13 (F6A4R) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

CST Logo
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using CXCL13 (F6A4R) Rabbit mAb.
Immunohistochemistry Image 2: CXCL13 (F6A4R) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using CXCL13 (F6A4R) Rabbit mAb.
Immunohistochemistry Image 3: CXCL13 (F6A4R) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human tonsil using CXCL13 (F6A4R) Rabbit mAb.
Immunohistochemistry Image 4: CXCL13 (F6A4R) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

CST Logo
Immunohistochemical analysis of paraffin-embedded normal human brain using CXCL13 (F6A4R) Rabbit mAb.
Immunohistochemistry Image 5: CXCL13 (F6A4R) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using CXCL13 (F6A4R) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 6: CXCL13 (F6A4R) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded 293T cell pellet, untransfected (left) or CXCL13-transfected (right), using CXCL13 (F6A4R) Rabbit mAb.
Immunohistochemistry Image 7: CXCL13 (F6A4R) Rabbit mAb
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.