Revision 1

#31743

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

orders@cellsignal.com

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P

Reactivity:
H M

Sensitivity:
Endogenous

MW (kDa):
81, 45-50, 20-25

Source/Isotype:
Rabbit IgG

UniProt ID:
#P11678

Entrez-Gene Id:
8288

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:500 - 1:2000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Eosinophil peroxidase (F4O7J) Rabbit mAb recognizes endogenous levels of total Eosinophil peroxidase protein. This antibody recognizes the full-length precursor, heavy chain, and light chain subunits of human Eosinophil peroxidase protein. This antibody is not recommended for immunohistochemical analysis of mouse tissues.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn585 of human Eosinophil peroxidase protein.

Background

Eosinophil peroxidase (EPX), also known as EPO, is a cationic protein stored in the secondary granulocytes of eosinophils (1-4). Unprocessed precursor EPX is an 80 kDa monomeric glycoprotein, which consists of two polypeptides linked by disulfide bonds: ~55 kDa heavy and ~15 kDa light chain (1-3, 6). In response to allergens or parasitic infection, eosinophils are recruited to affected tissues, where EPX catalyzes the formation of reactive oxidants lethal to tissue-invading parasites (1-4). In the tumor microenvironment of some breast cancers, eosinophils accumulate near blood vessels and infiltrate hypoxic regions, where EPX is released in high quantities by infiltrating immune cells (5). EPX may contribute to cancer progression by augmenting pro-tumorigenic collagen production and angiogenesis, making it a potential therapeutic target in breast cancer therapy (5).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: Human M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cells and tissues using Eosinophil peroxidase (F4O7J) Rabbit mAb (upper) or Histone H3 (D1H2) XP® Rabbit mAb #4499 (lower). Negative expression of Eosinophil peroxidase protein in DLD-1, Raji, and C2C12 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: Eosinophil peroxidase (F4O7J) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Eosinophil peroxidase protein (hEPX-Myc/DDK; +), using Eosinophil peroxidase (F4O7J) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: Eosinophil peroxidase (F4O7J) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Eosinophil peroxidase (F4O7J) Rabbit mAb.
Immunohistochemistry Image 1: Eosinophil peroxidase (F4O7J) Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Eosinophil peroxidase (F4O7J) Rabbit mAb.
Immunohistochemistry Image 2: Eosinophil peroxidase (F4O7J) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using Eosinophil peroxidase (F4O7J) Rabbit mAb.
Immunohistochemistry Image 3: Eosinophil peroxidase (F4O7J) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using Eosinophil peroxidase (F4O7J) Rabbit mAb.
Immunohistochemistry Image 4: Eosinophil peroxidase (F4O7J) Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human appendix using Eosinophil peroxidase (F4O7J) Rabbit mAb.
Immunohistochemistry Image 5: Eosinophil peroxidase (F4O7J) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human spleen using Eosinophil peroxidase (F4O7J) Rabbit mAb.
Immunohistochemistry Image 6: Eosinophil peroxidase (F4O7J) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human small intestine using Eosinophil peroxidase (F4O7J) Rabbit mAb.
Immunohistochemistry Image 7: Eosinophil peroxidase (F4O7J) Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human thymus using Eosinophil peroxidase (F4O7J) Rabbit mAb.
Immunohistochemistry Image 8: Eosinophil peroxidase (F4O7J) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded normal human esophagus using Eosinophil peroxidase (F4O7J) Rabbit mAb.
Immunohistochemistry Image 9: Eosinophil peroxidase (F4O7J) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using Eosinophil peroxidase (F4O7J) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 10: Eosinophil peroxidase (F4O7J) Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded MOLM-13 cell pellet (left, positive) or Raji cell pellet (right, negative) using Eosinophil peroxidase (F4O7J) Rabbit mAb.
Immunohistochemistry Image 11: Eosinophil peroxidase (F4O7J) Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.