Render Target: STATIC
Render Timestamp:
4/5/2025, 3:21:48 AM EDT
4/5/2025, 7:21:48 AM UTC
Commit: c91f970ca8df4f527662a05c7bd6e4d03c6fa173
XML generation date: 2025-04-01 12:56:09.674
Product last modified at: 2025-03-10T20:15:10.945Z
Cell Signaling Technology Logo

Basket Updated

0

Items added

1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Eosinophil peroxidase (F4O7J) Rabbit mAb #31743

Filter:
  • WB
  • IHC
Western Blotting Image 1: Eosinophil peroxidase (F4O7J) Rabbit mAb
Western blot analysis of extracts from various cells and tissues using Eosinophil peroxidase (F4O7J) Rabbit mAb (upper) or Histone H3 (D1H2) XP® Rabbit mAb #4499 (lower). Negative expression of Eosinophil peroxidase protein in DLD-1, Raji, and C2C12 cells is consistent with the predicted expression pattern.

To Purchase # 31743

Cat. # Size Qty. Price
31743T 20 µl
$172
31743S 100 µl
$402

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 81, 45-50, 20-25
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:500 - 1:2000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Eosinophil peroxidase (F4O7J) Rabbit mAb recognizes endogenous levels of total Eosinophil peroxidase protein. This antibody recognizes the full-length precursor, heavy chain, and light chain subunits of human Eosinophil peroxidase protein. This antibody is not recommended for immunohistochemical analysis of mouse tissues.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn585 of human Eosinophil peroxidase protein.

Background

Eosinophil peroxidase (EPX), also known as EPO, is a cationic protein stored in the secondary granulocytes of eosinophils (1-4). Unprocessed precursor EPX is an 80 kDa monomeric glycoprotein, which consists of two polypeptides linked by disulfide bonds: ~55 kDa heavy and ~15 kDa light chain (1-3, 6). In response to allergens or parasitic infection, eosinophils are recruited to affected tissues, where EPX catalyzes the formation of reactive oxidants lethal to tissue-invading parasites (1-4). In the tumor microenvironment of some breast cancers, eosinophils accumulate near blood vessels and infiltrate hypoxic regions, where EPX is released in high quantities by infiltrating immune cells (5). EPX may contribute to cancer progression by augmenting pro-tumorigenic collagen production and angiogenesis, making it a potential therapeutic target in breast cancer therapy (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.