View Featured Offers >>

InTraSeq Single Cell Analysis Resource Center

Graphic showing that InTraSeq™ Single Cell Analysis detects surface and intracellular proteins while guaranteeing a robust RNA signal, as opposed to other technologies attempting to capture intracellular protein in single-cell assays at the cost of RNA integrity

A) Most single-cell analysis techniques currently available detect RNA and use barcoded antibodies to measure surface proteins for phenotyping purposes. B) Many have tried developing methods to detect intracellular proteins, but these methods typically suffer from significant RNA loss and degradation. C) InTraSeq™ Single Cell Analysis detects RNA alongside intracellular and surface proteins while preserving RNA.

InTraSeq Single Cell Analysis (SCA): An Introduction

Developed and stringently validated by CST scientists, the proprietary formulation of InTraSeq (Intracellular protein and Transcriptomic Sequencing) enables unbiased quantitation of mRNA expression alongside intracellular and cell surface proteins at the single-cell level.

InTraSeq enables researchers to:

  • Detect RNA, intracellular, and cell surface proteins in thousands of cells simultaneously while guaranteeing robust RNA signal.
  • Investigate intracellular signaling pathways together with the transcriptome at the single-cell resolution.
  • Identify hard-to-detect cells with an unbiased depth-of-coverage.
  • Explore multiple molecular mechanisms in a single, streamlined experiment.

Below is a collection of resources to inform and support your single-cell analysis research with InTraSeq.

Cell Signaling Technology, CST, and InTraSeq are trademarks of Cell Signaling Technology, Inc. All rights reserved. 10x Genomics, 10x, Feature Barcode, and Chromium are the trademarks or registered trademarks of 10x Genomics, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Subject to patents licensed from 10x Genomics, Inc., for use with single-cell (i.e., Chromium) 10x products.