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InTraSeq 3' Conjugate Antibody Cocktail 1 #48167

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  • SCA
SCA Image 1: InTraSeq™ 3' Conjugate Antibody Cocktail 1
Human peripheral blood mononuclear cells (PBMCs) were processed in accordance with the InTraSeq™ 3’ protocol. The leftmost figures show the Uniform Manifold Approximation and Projection (UMAP) of the annotated PBMC clusters in each row. The top left figures display FeaturePlots of the TCF7 RNA and TCF1/TCF7 (C63D9) Rabbit mAb (InTraSeq™ 3' Conjugate 3013) #44070 protein expression levels. The top right figures display FeaturePlots of the AIF1 RNA and Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3003) #82746 protein expression levels. The bottom left figures display FeaturePlots of the FOXO1 RNA and FoxO1 (C29H4) Rabbit mAb (InTraSeq™ 3' Conjugate 3037) #45300 protein expression levels. The bottom right figures display FeaturePlots of the CD19 RNA and CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3010) #76014 protein expression levels. To generate similar plots, use the FeaturePlot command template from the Seurat computational package:
FeaturePlot(your_object, features = your_protein, max.cutoff = "q95", min.cutoff = "q5", order = TRUE) + scale_color_gradientn(colours = c("#22578B", "#73A9D2", "grey90", "#FDB393", "#b22222"))

To Purchase # 48167

Cat. # Size Qty. Price
48167S 22 µl
4 assays
$2,500
  • Product Includes
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Iba1/AIF-1 (E4O4W) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3003) #82746H MRabbit IgG
S6 Ribosomal Protein (54D2) Mouse mAb (InTraSeq™ 3' Conjugate 3005) #38889H MMouse IgG1
Phospho-CREB (Ser133) (87G3) Rabbit mAb (InTraSeq™ 3' Conjugate 3006) #95580H MRabbit IgG
GAPDH (14C10) Rabbit mAb (InTraSeq™ 3' Conjugate 3007) #27907H MRabbit IgG
CREB (48H2) Rabbit mAb (InTraSeq™ 3' Conjugate 3008) #41320H MRabbit IgG
CD19 (Intracellular Domain) (D4V4B) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3010) #76014H MRabbit IgG
Vimentin (D21H3) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3011) #96524H MRabbit IgG
TCF1/TCF7 (C63D9) Rabbit mAb (InTraSeq™ 3' Conjugate 3013) #44070H MRabbit IgG
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3014) #53639H MRabbit IgG
Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3015) #69098H MRabbit IgG
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (InTraSeq™ 3' Conjugate 3016) #79941H MRabbit IgG
Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (InTraSeq™ 3' Conjugate 3017) #53207H MRabbit IgG
Akt (pan) (C67E7) Rabbit mAb (InTraSeq™ 3' Conjugate 3018) #35123H MRabbit IgG
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3019) #92243H MRabbit IgG
Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3020) #27234H MRabbit IgG
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (InTraSeq™ 3' Conjugate 3021) #45003H MRabbit IgG
Stat3 (124H6) Mouse mAb (InTraSeq™ 3' Conjugate 3022) #68474H MMouse IgG2a kappa
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb (InTraSeq™ 3' Conjugate 3023) #76939H MRabbit IgG
Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3024) #92612H MRabbit IgG
NCAM1 (CD56) (E7X9M) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3025) #17655H MRabbit IgG
Ikaros (D6N9Y) Rabbit mAb (InTraSeq™ 3' Conjugate 3026) #42531H MRabbit IgG
Aiolos (D1C1E) Rabbit mAb (InTraSeq™ 3' Conjugate 3027) #61429H MRabbit IgG
Phospho-Stat3 (Ser727) (D4X3C) Rabbit mAb (InTraSeq™ 3' Conjugate 3031) #34774H MRabbit IgG
NFAT1 (D43B1) XP ® Rabbit mAb (InTraSeq™ 3' Conjugate 3032) #53722H MRabbit IgG
NF-κB p65 (D14E12) XP ® Rabbit mAb (InTraSeq™ 3' Conjugate 3033) #76695H MRabbit IgG
Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (InTraSeq™ 3' Conjugate 3034) #97410H MRabbit IgG
Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb (InTraSeq™ 3' Conjugate 3035) #25504H MRabbit IgG
Phospho-Glucocorticoid Receptor (Ser226) (D9D3V) Rabbit mAb (InTraSeq™ 3' Conjugate 3036) #25665H MRabbit IgG
FoxO1 (C29H4) Rabbit mAb (InTraSeq™ 3' Conjugate 3037) #45300H MRabbit IgG
Stat1 (D1K9Y) Rabbit mAb (InTraSeq™ 3' Conjugate 3038) #41599H MRabbit IgG
Phospho-Stat1 (Ser727) (D3B7) Rabbit mAb (InTraSeq™ 3' Conjugate 3039) #82887H MRabbit IgG

Product Information

Storage

This product is stable for at least 12 months when stored at -20ºC.

Protocol

Product Description

The InTraSeq™ methodology enables researchers to measure proteins (cytoplasmic, surface, and nuclear), including post-translational modifications (PTMs), along with RNA in their single-cell assays. While single-cell RNA sequencing (scRNAseq) has transformed the field of cellular and molecular biology, it only measures RNA transcripts within single cells. This presents a significant challenge, as RNA levels don't necessarily translate to protein abundance or activity. The InTraSeq technology addresses this limitation and can unravel missing information in scRNAseq experiments, uncovering new cell states and crucial biological data in single-cell analyses, especially since RNA does not reflect PTMs.

InTraSeq™ 3' Conjugate Antibody Cocktail 1 is a mixture of 31 titrated InTraSeq™ 3' Conjugates that are conjugated to 10x Genomics Feature Barcodes. This cocktail is only compatible with the InTraSeq™ 3' Assay Kit #82906 and is validated by CST using the 10x Genomics Chromium Single Cell 3' Kits with Feature Barcoding technology.

Specificity / Sensitivity


Species Reactivity:

Human, Mouse
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
10x Genomics, 10x, Feature Barcode, and Chromium are the trademarks or registered trademarks of 10x Genomics, Inc.
CST is a registered trademark of Cell Signaling Technology, Inc.
InTraSeq is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Subject to patents licensed from 10x Genomics, Inc. for use with single-cell (i.e., Chromium) 10x products.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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