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Render Timestamp: 2024-12-23T12:13:44.209Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-12-09 21:02:09.112
Product last modified at: 2024-11-19T09:00:26.565Z
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PDP - Template Name: InTraSeq Conjugate
PDP - Template ID: *******e148868
  1. Products /
  2. InTraSeq™ Kits & Reagents /
  3. p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (InTraSeq™ 3' Conjugate 3016)
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (InTraSeq™ 3' Conjugate 3016) #79941

Filter:
  • SCA

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • SCA-Single Cell Analysis 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Storage

    Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (InTraSeq™ 3' Conjugate 3016) detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. The antibody does not cross-react with JNK/SAPK or p38 MAP kinase.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of human p44 MAP kinase.

    Background

    Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli, including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.

    Database Links

    UniProt ID: P27361 , P28482


    Entrez-Gene Id: 5595 , 5594


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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