SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded head and neck carcinoma using TIM-3 (D5D5R™) & CO-00010-488 SignalStar™ Oligo-Antibody Pair #81365 (green), PD-1 (Intracellular Domain) (D4W2J) & CO-0008-594 SignalStar™ Oligo-Antibody Pair #35347 (yellow), PD-L1 (E1L3N®) & CO-0005-594 SignalStar™ Oligo-Antibody Pair #28249 (red), LAG3 (D2G4O™) & CO-0026-750 SignalStar™ Oligo-Antibody Pair #85452 (cyan), CD3ε (D7A6E™) & CO-0001-488 SignalStar™ Oligo-Antibody Pair #92856 (pink), CD68 (D4B9C) & CO-0007-594 SignalStar™ Oligo-Antibody Pair #77318 (orange), CD8α (D8A8Y) & CO-0004-647 SignalStar™ Oligo-Antibody Pair #66676 (magenta),Pan-Keratin (C11) & CO-0003-750 SignalStar™ Oligo-Antibody Pair #97227 (grey), CD206/MRC1 (E6T5J) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #59414 (teal), CD20 (E7B7T) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #83399 (lavender), CD45 (Intracellular Domain) (D9M8I) XP® Rabbit mAb (Alexa Fluor® 750 Conjugate) (olive), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Context matters. Spatial biology uses techniques such as multiplex immunohistochemistry (mIHC) to identify which cells are present and how they behave within their tissue environment. Multiplexing 2-12 targets at a time provides a full picture on how and where critical biological events occur, helping researchers understand disease progression and identify biomarker co-expression.
CST offers a diverse portfolio of easy-to-use, flexible, and validated multiplex assay solutions that are specific to the number of targets you want to monitor. Performing a 2-plex or 3-plex experiment? IHC-validated direct conjugate antibodies will deliver reliable results you trust. Need 3-plex to 8-plex capabilities? SignalStar™ mIHC is the solution for you. Want to get the most out of your tissue? You’ll be able to multiplex 8+ targets using a combination of SignalStar technology, IHC-validated direct conjugates, and the fluorescence removal kit.
We have organized a collection of mIHC resources, organized by the plex that meets your needs, to help you get data you need while maintaining spatial context and tissue integrity.
