DAPI #4083
- IF
- F
Product Information
Product Usage Information
Directions for Use: DAPI is supplied as a lyophilized powder. For a 20 mg/ml stock, reconstitute the 1 mg in 50 µl deionized water or dimethylformamide. Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection:
Immunofluorescence: Counterstain with DAPI as the final step in your staining procedure. Rinse samples twice in PBS for five min each. Dilute DAPI stock solution to a concentration between 1 - 0.1 µg/ml in PBS and incubate for 5 min at room temperature in the dark. Rinse samples once in PBS and then prepare for imaging. Examine immediately using appropriate excitation wavelength.
Alternatively, dilute DAPI stock solution to a concentration between 1 - 0.1 µg/ml in mounting media, apply to cells, and prepare for imaging. Examine immediately using appropriate excitation wavelength.
Flow Cytometry: Rinse samples once in Incubation Buffer. Dilute DAPI stock solution to a concentration between 1.60-0.400 µg/ml in PBS and incubate for 15 min at room temperature in the dark before analyzing cells on flow cytometer.
Storage
Fluorescent Properties
- Free dye excitation maximum = 340 nm
- Free dye emission maximum = 488 nm
- DNA complex excitation maximum = 364 nm
- DNA complex emission maximum = 454 nm
Product Description
| Molecular Formula | C16H15N5 2(HCl) |
Background
Limited Uses
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