Revision 3
#15171
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IHC-P, IF-IC, FC-FP
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
25
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q92520
Entrez-Gene Id:
10447
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunohistochemistry (Paraffin) | 1:300 |
Immunofluorescence (Immunocytochemistry) | 1:400 |
Flow Cytometry (Fixed/Permeabilized) | 1:200 |
Storage
Specificity/Sensitivity
FAM3C (D1S2D) XP® Rabbit mAb recognizes endogenous levels of total FAM3C protein. Based on the sequence of the immunogenic peptide, the antibody is not expected to cross-react with other FAM3 family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly169 of human FAM3C protein.
Background
FAM3C, also known as ILEI (interleukin-like epithelial-to-mesenchymal transition [EMT] inducer), is a cytokine-like protein and member of the FAM3 family. FAM3C plays an important role in EMT and metastasis during cancer progression in human and mouse cells, and is highly expressed in human cancer (1,2). In colorectal cancer, researchers have indicated that FAM3C is a marker for EMT and tumor progression, and that high expression of FAM3C is predictive of poor prognosis (3). While EMT induction by FAM3C can be independent of TGF-beta, research studies have also shown TGF-beta-dependent regulation of FAM3C expression at the translational level in mouse and human cells (4,5).
FAM3C has also been linked to regulation of osteoblast differentiation (6), and to accumulation of amyloid beta plaques in Alzheimer’s disease (7). FAM3C exists in monomeric and in homodimeric form, and research shows that FAM3C homodimers contain its EMT-inducing and tumor promoting activity (8).
Background References
- Waerner, T. et al. (2006) Cancer Cell 10, 227-39.
- Lahsnig, C. et al. (2009) Oncogene 28, 638-50.
- Gao, Z.H. et al. (2014) Histopathology 65, 527-38.
- Chaudhury, A. et al. (2010) Nat Cell Biol 12, 286-93.
- Song, Q. et al. (2014) Tumour Biol 35, 1377-82.
- Bendre, A. et al. Differentiation 93, 50-57.
- Liu, L. et al. (2016) Neuroscience 330, 236-46.
- Kral, M. et al. (2017) FEBS J 284, 3484-3505.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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