WB, IP, IHC-P
H
Endogenous
32, 42
Rabbit IgG
#O00214
3964
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro176 of human Galectin-8/LGALS8 protein.
Background
Galectins are a family of β-galactose binding proteins with intracellular and extracellular activities regulating cell adhesion and migration, immune responses, inflammation, cell death, and proliferation and are linked to several pathological conditions (reviewed in 1). Galectins can be divided into three groups: 1) prototype galectins (Gal-1, -2, -5, -7, -10, -11, -13, and -16) having one carbohydrate-recognition domain (CRD); 2) tandem-repeat type galectins (Gal-4, -6, -8, -9, and -12) that have two CRDs joined by a linker peptide; and 3) a chimera-type (Gal-3) that has a single CRD joined to an N-terminal non-lectin domain.
Galectin-8 (Gal-8; gene name LGALS8), which belongs to the group of tandem-repeat galectins, is important in adaptive and innate immunity with significant roles in host defense as well as tumor progression (reviewed in 2,3). Extracellular galectin-8 promotes cell adhesion through interaction with several cell surface proteins, including integrins (4,5), CD44 (6), CD45 (7), and CD166 (8). Intracellular galectin-8 binds to farnesylated K-Ras4B, regulating MAPK signaling (9). It also exerts a role in xenophagy by binding to glycans exposed on bacterial pathogens and recruiting NDP52 for destruction by autophagy, thereby restricting bacterial proliferation (10,11). Abundant expression of galectin-8 has been observed in various cancer types and, in some cases, associated with poor prognosis (12-14).
- Johannes, L. et al. (2018) J Cell Sci 131, jcs208884. doi: 10.1242/jcs.208884.
- Purić, E. et al. (2024) Med Res Rev, doi: 10.1002/med.22041.
- Zick, Y. (2022) Biochem Soc Trans 50, 135-149.
- Cárcamo, C. et al. (2006) Exp Cell Res 312, 374-86.
- Hadari, Y.R. et al. (2000) J Cell Sci 113 ( Pt 13), 2385-97.
- Eshkar Sebban, L. et al. (2007) J Immunol 179, 1225-35.
- Tribulatti, M.V. et al. (2009) J Leukoc Biol 86, 371-80.
- Delgado, V.M. et al. (2011) FASEB J 25, 242-54.
- Meinohl, C. et al. (2019) Cancers (Basel) 12, 30. doi: 10.3390/cancers12010030.
- Thurston, T.L. et al. (2012) Nature 482, 414-8.
- Kim, B.W. et al. (2013) Nat Commun 4, 1613.
- Wang, Y. et al. (2023) Am J Cancer Res 13, 2517-2529.
- Gentilini, L.D. et al. (2017) Oncotarget 8, 44654-44668.
- Elola, M.T. et al. (2014) Histol Histopathol 29, 1093-105.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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