Revision 1
#52445
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IHC-P
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
35, 85
Source/Isotype:
Rabbit IgG
UniProt ID:
#P14210
Entrez-Gene Id:
3082
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunohistochemistry (Paraffin) | 1:200 |
Storage
Specificity/Sensitivity
HGF β (D6S7D) XP® Rabbit mAb recognizes endogenous levels of total HGF protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human HGF protein.
Background
The Hepatocyte Growth Factor (HGF, also known as Scatter Factor) was initially discovered as a mitogenic cytokine that induced hepatocyte replication and proliferation (1-3). HGF is produced by stromal cells where it is processed by extracellular serine proteases into a heterodimer consisting of alpha and beta subunits (4). Through activation of its receptor, cMET, HGF has a wide range of effects beyond hepatocytes that includes angiogenesis, epithelial cell proliferation and morphogenesis, and tissue protection and regeneration (5). The HGF-cMET axis has been associated with several diseases, including cancer, where HGF has been shown to promote invasion, metastasis, and drug resistance (6,7). These research studies suggest that HGF is a potential diagnostic and therapeutic target.
Background References
- Nakamura, T. et al. (1984) Biochem Biophys Res Commun 122, 1450-9.
- Russell, W.E. et al. (1984) J Cell Physiol 119, 183-92.
- Gohda, E. et al. (1988) J Clin Invest 81, 414-9.
- Kataoka, H. et al. Cancer Metastasis Rev 22, 223-36.
- Nakamura, T. and Mizuno, S. (2010) Proc Jpn Acad Ser B Phys Biol Sci 86, 588-610.
- Matsumoto, K. and Nakamura, T. (2006) Int J Cancer 119, 477-83.
- Yano, S. et al. (2008) Cancer Res 68, 9479-87.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.