HGF β (D6S7D) XP® Rabbit mAb #52445
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35, 85 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 |
Storage
Protocol
Specificity / Sensitivity
HGF β (D6S7D) XP® Rabbit mAb recognizes endogenous levels of total HGF protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human HGF protein.
Background
The Hepatocyte Growth Factor (HGF, also known as Scatter Factor) was initially discovered as a mitogenic cytokine that induced hepatocyte replication and proliferation (1-3). HGF is produced by stromal cells where it is processed by extracellular serine proteases into a heterodimer consisting of alpha and beta subunits (4). Through activation of its receptor, cMET, HGF has a wide range of effects beyond hepatocytes that includes angiogenesis, epithelial cell proliferation and morphogenesis, and tissue protection and regeneration (5). The HGF-cMET axis has been associated with several diseases, including cancer, where HGF has been shown to promote invasion, metastasis, and drug resistance (6,7). These research studies suggest that HGF is a potential diagnostic and therapeutic target.
- Nakamura, T. et al. (1984) Biochem Biophys Res Commun 122, 1450-9.
- Russell, W.E. et al. (1984) J Cell Physiol 119, 183-92.
- Gohda, E. et al. (1988) J Clin Invest 81, 414-9.
- Kataoka, H. et al. Cancer Metastasis Rev 22, 223-36.
- Nakamura, T. and Mizuno, S. (2010) Proc Jpn Acad Ser B Phys Biol Sci 86, 588-610.
- Matsumoto, K. and Nakamura, T. (2006) Int J Cancer 119, 477-83.
- Yano, S. et al. (2008) Cancer Res 68, 9479-87.
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