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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IF-IC, FC-FP, FC-L
Reactivity:
H
Sensitivity:
Endogenous
MW (kDa):
60-80
Source/Isotype:
Rabbit IgG
UniProt ID:
#P31785
Entrez-Gene Id:
3561
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:100 |
Flow Cytometry (Live) | 1:50 - 1:100 |
Storage
Specificity/Sensitivity
IL-2Rγ (E1V1O) Rabbit mAb recognizes endogenous levels of total IL-2Rγ protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human IL-2Rγ protein.
Background
IL-2Rγ (Cytokine receptor common subunit gamma, γc) is part of the interleukin-2 (IL-2) receptor, comprised of alpha, beta, and gamma chains (1-6). The gamma chain is required for the formation of high- and intermediate-affinity receptors, which consist of alpha beta gamma heterotrimers and beta gamma heterodimers (1). IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 all share the same common gamma chain, which was originally discovered as the gamma chain of the IL-2 receptor (3-6). The γ chain family of cytokines contribute to lymphocyte homeostasis by controlling aspects of lymphocyte development, growth, differentiation, and survival (5,6). In humans, a mutation in IL-2Rγ results in X-linked severe combined immunodeficiency (XSCID), which is a disease characterized by the absence of T and NK cells, with nonfunctional B cells (2).
Background References
- Takeshita, T. et al. (1992) Science 257, 379-82.
- Noguchi, M. et al. (1993) Cell 73, 147-57.
- Russell, S.M. et al. (1993) Science 262, 1880-3.
- Noguchi, M. et al. (1993) Science 262, 1877-80.
- Rochman, Y. et al. (2009) Nat Rev Immunol 9, 480-90.
- Lin, J.X. and Leonard, W.J. (2018) Cold Spring Harb Perspect Biol 10, a028449. doi: 10.1101/cshperspect.a028449.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
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