IL-2Rγ (E1V1O) Rabbit mAb #49622
- WB
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60-80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:100 |
| Flow Cytometry (Live) | 1:50 - 1:100 |
Storage
Protocol
Specificity / Sensitivity
IL-2Rγ (E1V1O) Rabbit mAb recognizes endogenous levels of total IL-2Rγ protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human IL-2Rγ protein.
Background
IL-2Rγ (Cytokine receptor common subunit gamma, γc) is part of the interleukin-2 (IL-2) receptor, comprised of alpha, beta, and gamma chains (1-6). The gamma chain is required for the formation of high- and intermediate-affinity receptors, which consist of alpha beta gamma heterotrimers and beta gamma heterodimers (1). IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21 all share the same common gamma chain, which was originally discovered as the gamma chain of the IL-2 receptor (3-6). The γ chain family of cytokines contribute to lymphocyte homeostasis by controlling aspects of lymphocyte development, growth, differentiation, and survival (5,6). In humans, a mutation in IL-2Rγ results in X-linked severe combined immunodeficiency (XSCID), which is a disease characterized by the absence of T and NK cells, with nonfunctional B cells (2).
- Takeshita, T. et al. (1992) Science 257, 379-82.
- Noguchi, M. et al. (1993) Cell 73, 147-57.
- Russell, S.M. et al. (1993) Science 262, 1880-3.
- Noguchi, M. et al. (1993) Science 262, 1877-80.
- Rochman, Y. et al. (2009) Nat Rev Immunol 9, 480-90.
- Lin, J.X. and Leonard, W.J. (2018) Cold Spring Harb Perspect Biol 10, a028449. doi: 10.1101/cshperspect.a028449.
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