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#58595
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IF-F, IF-IC
Reactivity:
H M R
Sensitivity:
Endogenous
MW (kDa):
270
Source/Isotype:
Rabbit
UniProt ID:
#P46821
Entrez-Gene Id:
4131
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunofluorescence (Frozen) | 1:3200 |
Immunofluorescence (Immunocytochemistry) | 1:3200 - 1:6400 |
Storage
Specificity/Sensitivity
MAP1B Antibody recognizes endogenous levels of total MAP1B protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1570 of human MAP1B protein. Antibodies are purified by peptide affinity chromatography.
Background
Microtubule-associated protein 1B (MAP1B) is a member of the high molecular weight microtubule binding phosphoprotein family, along with MAP1A (1). MAP1B is highly expressed in neurons, predominantly during development, where it regulates axonal guidance and elongation through its influence on microtubule dynamics (1,2). In mature neurons, MAP1B plays a role in regulation and structure of dendritic spines in glutamatergic synapses. MAP1B deficient neurons have been shown to display structural presynaptic deficiencies, including a decrease in density at synaptic terminals and disruption of synaptic vesicle fusion events (2). Mutations in MAP1B have been linked to developmental disorders affecting cognitive ability through a deficit in white matter and compromised axons (3).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IF-F: Immunofluorescence (Frozen)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
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Limited Uses
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