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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

MAP1B Antibody #58595

Filter:
  • WB
  • IF
Western Blotting Image 1: MAP1B Antibody
Western blot analysis of extracts from A549 cells, NB-4 cells, and mouse brain tissue using MAP1B Antibody (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Negative expression of MAP1B protein in NB-4 cells is consistent with the predicted expression pattern.

To Purchase # 58595

Cat. # Size Qty. Price
58595S 100 µl
$306

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 270
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Frozen) 1:3200
Immunofluorescence (Immunocytochemistry) 1:3200 - 1:6400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MAP1B Antibody recognizes endogenous levels of total MAP1B protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1570 of human MAP1B protein. Antibodies are purified by peptide affinity chromatography.

Background

Microtubule-associated protein 1B (MAP1B) is a member of the high molecular weight microtubule binding phosphoprotein family, along with MAP1A (1). MAP1B is highly expressed in neurons, predominantly during development, where it regulates axonal guidance and elongation through its influence on microtubule dynamics (1,2). In mature neurons, MAP1B plays a role in regulation and structure of dendritic spines in glutamatergic synapses. MAP1B deficient neurons have been shown to display structural presynaptic deficiencies, including a decrease in density at synaptic terminals and disruption of synaptic vesicle fusion events (2). Mutations in MAP1B have been linked to developmental disorders affecting cognitive ability through a deficit in white matter and compromised axons (3).

Pathways

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