WB
H M Mk
Endogenous
28
Rabbit IgG
#Q9UBP6
4234
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala55 of human METTL1 protein.
Background
METTL1 is a conserved methyltransferase that forms a complex with WDR4 to catalyze 7-methylguanosine (m7G) on mRNA, tRNA, and miRNAs (1,2). Both METTL1 and WDR4 are required for m7G modification, with METTL1 being the catalytic component and WDR4 stabilizing the complex (2). m7G on mRNA is required for translation and stem cell differentiation, while m7G on miRNA can regulate structure and biogenesis (3,4). METTL1 is phosphorylated by PKB and RSK, which results in its inactivation (5). Overexpression of METTL1 has been shown to be oncogenic and contribute to poor prognosis in various tumor types (6-8).
- Bahr, A. et al. (1999) Genomics 57, 424-8.
- Alexandrov, A. et al. (2002) RNA 8, 1253-66.
- Lin, S. et al. (2018) Mol Cell 71, 244-255.e5.
- Pandolfini, L. et al. (2019) Mol Cell 74, 1278-1290.e9.
- Cartlidge, R.A. et al. (2005) EMBO J 24, 1696-705.
- Tian, Q.H. et al. (2019) J Mol Med (Berl) 97, 1535-1545.
- Li, L. et al. (2021) Cancer Cell Int 21, 633.
- Ying, X. et al. (2021) Clin Transl Med 11, e675.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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