Revision 5
#58418
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP, IHC-P, ChIP, ChIP-seq, C&R
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
80
Source/Isotype:
Rabbit IgG
UniProt ID:
#O95251
Entrez-Gene Id:
11143
Product Usage Information
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Immunohistochemistry (Paraffin) | 1:1000 |
Chromatin IP | 1:50 |
Chromatin IP-seq | 1:50 |
CUT&RUN | 1:50 |
Storage
Specificity/Sensitivity
MYST2 (D4N3F) Rabbit mAb recognizes endogenous levels of total MYST2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Val222 of human MYST2 protein.
Background
MYST2, also known as HBO1 and lysine acetyltransferase 7 (KAT7), is a member of the MYST (MOZ, YBF2, SAS2 and Tip60) family of histone acetyltransferases (HATs). MYST2 is the catalytic subunit of the HBO1 acetyltransferase complex, which consists of MYST2, MYST/ESA1-associated factor 6 (MEAF6), inhibitor of growth protein 4 (ING4) or inhibitor of growth protein 5 (ING5), and one of two families of scaffold proteins (JADE-1/2/3 or BRPF1/2/3) (1,2). The substrate specificity of the HBO1 complex is determined by the associated scaffold protein. HBO1 complexes containing JADE scaffold proteins acetylate histone H4 on lysines 5, 8 and 12, while complexes containing BRPF scaffold proteins acetylate histone H3 on lysines 14 and 23 (2). In addition, the scaffold protein appears to regulate the function of the HBO1 complex. Complexes containing JADE scaffold proteins bind to origin recognition complex 1 (ORC1) and regulate licensing of DNA replication, while HBO1 complexes containing BRPF scaffold proteins regulate transcription (2-5). MYST2 is required for regulation of cell proliferation (1), adipogenesis (6), embryonic development (7) and survival of fetal liver erythroblasts (8). In addition, MYST2 is over-expressed in several human cancers, including cancers of the testis, ovary, breast, stomach, esophagus, and bladder (9). The MYST2 gene is amplified and protein is over-expressed in breast cancers, and over-expression of MYST2 increases anchorage-independent growth of several breast cancer cell lines (10).
Background References
- Avvakumov, N. et al. (2012) Mol Cell Biol 32, 689-703.
- Lalonde, M.E. et al. (2013) Genes Dev 27, 2009-24.
- Iizuka, M. and Stillman, B. (1999) J Biol Chem 274, 23027-34.
- Iizuka, M. et al. (2006) Mol Cell Biol 26, 1098-108.
- Miotto, B. and Struhl, K. (2010) Mol Cell 37, 57-66.
- Johmura, Y. et al. (2008) J Biol Chem 283, 2265-74.
- Kueh, A.J. et al. (2011) Mol Cell Biol 31, 845-60.
- Mishima, Y. et al. (2011) Blood 118, 2443-53.
- Iizuka, M. et al. (2009) Gene 436, 108-14.
- Hu, X. et al. (2009) Mol Cancer Res 7, 511-22.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) ChIP: Chromatin IP C&R: CUT&RUN
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a registered trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 5
#58418
MYST2 (D4N3F) Rabbit mAb
Western blot analysis of extracts from various cell lines using MYST2 (D4N3F) Rabbit mAb.
Immunoprecipitation of MYST2 from MOLT4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MYST2 (D4N3F) Rabbit mAb. Western blot analysis was performed using MYST2 (D4N3F) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MYST2 (D4N3F) Rabbit mAb.
Revision 5
#58418
MYST2 (D4N3F) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MYST2 (D4N3F) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using MYST2 (D4N3F) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 cells and MYST2 (D4N3F) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across PRCC gene. For additional ChIP-seq tracks, please download the product data sheet.
Revision 5
#58418
MYST2 (D4N3F) Rabbit mAb
Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 cells and MYST2 (D4N3F) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across chromosome 1 (upper), including PRCC (lower).
Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 cells and either MYST2 (D4N3F) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA9 Promoter Primers #14909, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
CUT&RUN was performed with HCT116 cells and MYST2 (D4N3F) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA library was prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figure shows binding across ME1 gene, a known target gene of MYST2 (see additional figure containing CUT&RUN-qPCR data).
Revision 5
#58418
MYST2 (D4N3F) Rabbit mAb
CUT&RUN was performed with HCT116 cells and MYST2 (D4N3F) Rabbit mAb, using CUT&RUN Assay Kit #86652. DNA library was prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 6 (upper), including ME1 (lower), a known target gene of MYST2 (see additional figure containing CUT&RUN-qPCR data).
CUT&RUN was performed with HCT116 cells and MYST2 (D4N3F) Rabbit mAb or Rabbit (DA1E) mAb IgG XP® Isotype Control (CUT&RUN) #66362, using CUT&RUN Assay Kit #86652. The enriched DNA was quantified by real-time PCR using human ME1 promoter primers and human PANTR1 promoter primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.