MYST2 (D4N3F) Rabbit mAb #58418
- WB
- IP
- IHC
- ChIP
- C&R
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:1000 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
| CUT&RUN | 1:50 |
Storage
Protocol
Specificity / Sensitivity
MYST2 (D4N3F) Rabbit mAb recognizes endogenous levels of total MYST2 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein surrounding Val222 of human MYST2 protein.
Background
MYST2, also known as HBO1 and lysine acetyltransferase 7 (KAT7), is a member of the MYST (MOZ, YBF2, SAS2 and Tip60) family of histone acetyltransferases (HATs). MYST2 is the catalytic subunit of the HBO1 acetyltransferase complex, which consists of MYST2, MYST/ESA1-associated factor 6 (MEAF6), inhibitor of growth protein 4 (ING4) or inhibitor of growth protein 5 (ING5), and one of two families of scaffold proteins (JADE-1/2/3 or BRPF1/2/3) (1,2). The substrate specificity of the HBO1 complex is determined by the associated scaffold protein. HBO1 complexes containing JADE scaffold proteins acetylate histone H4 on lysines 5, 8 and 12, while complexes containing BRPF scaffold proteins acetylate histone H3 on lysines 14 and 23 (2). In addition, the scaffold protein appears to regulate the function of the HBO1 complex. Complexes containing JADE scaffold proteins bind to origin recognition complex 1 (ORC1) and regulate licensing of DNA replication, while HBO1 complexes containing BRPF scaffold proteins regulate transcription (2-5). MYST2 is required for regulation of cell proliferation (1), adipogenesis (6), embryonic development (7) and survival of fetal liver erythroblasts (8). In addition, MYST2 is over-expressed in several human cancers, including cancers of the testis, ovary, breast, stomach, esophagus, and bladder (9). The MYST2 gene is amplified and protein is over-expressed in breast cancers, and over-expression of MYST2 increases anchorage-independent growth of several breast cancer cell lines (10).
- Avvakumov, N. et al. (2012) Mol Cell Biol 32, 689-703.
- Lalonde, M.E. et al. (2013) Genes Dev 27, 2009-24.
- Iizuka, M. and Stillman, B. (1999) J Biol Chem 274, 23027-34.
- Iizuka, M. et al. (2006) Mol Cell Biol 26, 1098-108.
- Miotto, B. and Struhl, K. (2010) Mol Cell 37, 57-66.
- Johmura, Y. et al. (2008) J Biol Chem 283, 2265-74.
- Kueh, A.J. et al. (2011) Mol Cell Biol 31, 845-60.
- Mishima, Y. et al. (2011) Blood 118, 2443-53.
- Iizuka, M. et al. (2009) Gene 436, 108-14.
- Hu, X. et al. (2009) Mol Cancer Res 7, 511-22.
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