Revision 1
#74384
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877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IF-F, IF-IC
Reactivity:
H M Mk
Sensitivity:
Endogenous
MW (kDa):
300
Source/Isotype:
Rabbit IgG
UniProt ID:
#B2RWS6
Entrez-Gene Id:
328572
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Supplied in 1X PBS, BSA and Azide Free.
For standard formulation of this product see product #67621
Storage
Specificity/Sensitivity
p300 (E6D1T) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total p300 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p300 protein.
Background
CBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). Phosphorylation of p300 at Ser89 by PKC represses its transcriptional activity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors (3,4). Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ (5). Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex (6). CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS (7). The role of acetylation of CBP/p300 is of particular interest (2,8). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (9).
Background References
- Goodman, R.H. and Smolik, S. (2000) Genes Dev 14, 1553-77.
- Chan, H.M. and La Thangue, N.B. (2001) J. Cell Sci. 114, 2363-2373.
- Yuan, L.W. and Gambee, J.E. (2000) J. Biol. Chem. 275, 40946-40951.
- Yang, W. et al. (2001) J. Biol. Chem. 276, 38341-38344.
- Guo, S. et al. (2001) J. Biol. Chem. 276, 8516-8523.
- Zanger, K. et al. (2001) Mol. Cell 7, 551-558.
- Impey, S. et al. (2002) Neuron 34, 235-244.
- Yuan, L.W. and Giordano, A. (2002) Oncogene 21, 2253-2260.
- Thompson, P.R. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
W: Western Blotting IF-F: Immunofluorescence (Frozen)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
Western blot analysis of extracts from control NIH/3T3 cells (lane 1) or p300 knockout NIH/3T3 cells (lane 2) using p300 (E6D1T) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). The absence of signal in the p300 knockout NIH/3T3 cells confirms specificity of the antibody for p300. Data were generated using the standard formulation of this product.
Western blot analysis of extracts from various cell lines using p300 (E6D1T) Rabbit mAb. Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of mouse liver using p300 (E6D1T) Rabbit mAb (red), Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) #8617 (green), and S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (pseudocolor blue). Data were generated using the standard formulation of this product.
Revision 1
Confocal immunofluorescent analysis of mouse kidney using p300 (E6D1T) Rabbit mAb (red), Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) #8617 (green), and S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (pseudocolor blue). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of control NIH/3T3 cells (left, positive) or p300 knockout NIH/3T3 cells (right, negative) using p300 (E6D1T) Rabbit mAb (green), DyLight™ 650 Phalloidin #12956 (red), and DAPI #4083 (blue). Data were generated using the standard formulation of this product.