p300 (E6D1T) Rabbit mAb (BSA and Azide Free) #74384
- WB
- IF
Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 300 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
p300 (E6D1T) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total p300 protein.
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p300 protein.
Background
CBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). Phosphorylation of p300 at Ser89 by PKC represses its transcriptional activity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors (3,4). Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ (5). Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex (6). CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS (7). The role of acetylation of CBP/p300 is of particular interest (2,8). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (9).
- Goodman, R.H. and Smolik, S. (2000) Genes Dev 14, 1553-77.
- Chan, H.M. and La Thangue, N.B. (2001) J. Cell Sci. 114, 2363-2373.
- Yuan, L.W. and Gambee, J.E. (2000) J. Biol. Chem. 275, 40946-40951.
- Yang, W. et al. (2001) J. Biol. Chem. 276, 38341-38344.
- Guo, S. et al. (2001) J. Biol. Chem. 276, 8516-8523.
- Zanger, K. et al. (2001) Mol. Cell 7, 551-558.
- Impey, S. et al. (2002) Neuron 34, 235-244.
- Yuan, L.W. and Giordano, A. (2002) Oncogene 21, 2253-2260.
- Thompson, P.R. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.
Limited Uses
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