WB, IP
H
Endogenous
40
Rabbit IgG
#O43561
27040
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr161 of human LAT protein, long isoform.
Background
LAT, a transmembrane adaptor protein expressed in T, NK, and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr220 enables the binding of Grb2, Gads/SLP-76, PLCγ1, and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4).
Research studies have shown that Zap-70 phosphorylates LAT at Tyr161, which creates an additional docking site to facilitate PLCγ1 recruitment, phosphorylation, and activation (3-5). Tyr161 is a relatively poor substrate for Zap-70 and following TCR engagement, phosphorylation of LAT at Tyr161 is delayed relative to the other distal tyrosine residues. Given the importance of PLCγ1 in regulating multiple T cell effector functions, it is posited that the delayed kinetics of Tyr161 phosphorylation serves as molecular rheostat in order to allow the TCR to effectively discriminate between autoantigens and foreign antigens (6,7).
- Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-83.
- Zhang, W. et al. (1998) Cell 92, 83-92.
- Paz, P. E. et al. (2001) Biochem. J. 356, 461-71.
- Zhang, W. et al. (2000) J. Biol. Chem. 275, 23355-61.
- Zeng, L. et al. (2021) J Cell Biol 220, e202009154. doi: 10.1083/jcb.202009154.
- Houtman, J.C. et al. (2005) J Immunol 175, 2449-58.
- Lo, W.L. et al. (2019) Nat Immunol 20, 1481-1493.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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