Phospho-LAT (Tyr161) (E9Q2R) Rabbit mAb #88077
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Research studies have shown that Zap-70 phosphorylates LAT at Tyr161, which creates an additional docking site to facilitate PLCγ1 recruitment, phosphorylation, and activation (3-5). Tyr161 is a relatively poor substrate for Zap-70 and following TCR engagement, phosphorylation of LAT at Tyr161 is delayed relative to the other distal tyrosine residues. Given the importance of PLCγ1 in regulating multiple T cell effector functions, it is posited that the delayed kinetics of Tyr161 phosphorylation serves as molecular rheostat in order to allow the TCR to effectively discriminate between autoantigens and foreign antigens (6,7).
- Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-83.
- Zhang, W. et al. (1998) Cell 92, 83-92.
- Paz, P. E. et al. (2001) Biochem. J. 356, 461-71.
- Zhang, W. et al. (2000) J. Biol. Chem. 275, 23355-61.
- Zeng, L. et al. (2021) J Cell Biol 220, e202009154. doi: 10.1083/jcb.202009154.
- Houtman, J.C. et al. (2005) J Immunol 175, 2449-58.
- Lo, W.L. et al. (2019) Nat Immunol 20, 1481-1493.
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