Revision 1
#33210
Store at -20C
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IF-IC
Reactivity:
H Mk
Sensitivity:
Endogenous
MW (kDa):
68
Source/Isotype:
Rabbit IgG
UniProt ID:
#Q07666
Entrez-Gene Id:
10657
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Specificity/Sensitivity
SAM68 (E6Y2F) Rabbit mAb recognizes endogenous levels of total SAM68 protein. This antibody does not cross-react with KHDRBS2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly402 of human SAM68 protein.
Background
Src associated in mitosis 68 kDa (SAM68) is a member of the signal transduction and activation of RNA (STAR) family of RNA binding proteins (1,2). SAM68 is a multi-functional protein with roles in signal transduction, cell cycle regulation, transcription, and alternative splicing (1-3). SAM68 acts as a scaffold protein mediating responses to various stimuli that activate receptors, such as the T cell receptor and the insulin receptor, linking activation of various signal transduction pathways with post-transcriptional gene regulation (3). SAM68 is a target for phosphorylation by Src-related kinases and Erk1/2 mitogen-activated protein kinases. Phosphorylation of SAM68 regulates both its affinity for RNA and alternative splicing of RNAs like CD44 receptor mRNA (1,4). Many of the activities of SAM68 and its targets for alternative splicing have been implicated in the development and progression of spinal muscular atrophy and cancers such as prostate and breast carcinomas, where SAM68 is frequently upregulated (1,5-8).
Background References
- Bielli, P. et al. (2011) Endocr Relat Cancer 18, R91-R102.
- Lukong, K.E. and Richard, S. (2003) Biochim Biophys Acta 1653, 73-86.
- Najib, S. et al. (2005) Cell Mol Life Sci 62, 36-43.
- Matter, N. et al. (2002) Nature 420, 691-5.
- Pedrotti, S. et al. (2010) EMBO J 29, 1235-47.
- Busà, R. et al. (2007) Oncogene 26, 4372-82.
- Rajan, P. et al. (2008) J Pathol 215, 67-77.
- Song, L. et al. (2010) J Pathol 222, 227-37.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: Human M: Mouse R: Rat Hm: Hamster Mk: Monkey Vir: Virus Mi: Mink C: Chicken Dm: D. melanogaster X: Xenopus Z: Zebrafish B: Bovine Dg: Dog Pg: Pig Sc: S. cerevisiae Ce: C. elegans Hr: Horse GP: Guinea Pig Rab: Rabbit G: Goat All: All Species Expected
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.
Revision 1
#33210
SAM68 (E6Y2F) Rabbit mAb
Western blot analysis of extracts from various cell lines using SAM68 (E6Y2F) Rabbit mAb.
Confocal immunofluorescent analysis of HCT 116 cells, mock transfected (left, high-expressing) or transfected with siRNA directed against human SAM68 (right, low-expressing), using SAM68 (E6Y2F) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).