SAM68 (E6Y2F) Rabbit mAb #33210
- WB
- IF
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 68 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Protocol
Specificity / Sensitivity
SAM68 (E6Y2F) Rabbit mAb recognizes endogenous levels of total SAM68 protein. This antibody does not cross-react with KHDRBS2 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly402 of human SAM68 protein.
Background
Src associated in mitosis 68 kDa (SAM68) is a member of the signal transduction and activation of RNA (STAR) family of RNA binding proteins (1,2). SAM68 is a multi-functional protein with roles in signal transduction, cell cycle regulation, transcription, and alternative splicing (1-3). SAM68 acts as a scaffold protein mediating responses to various stimuli that activate receptors, such as the T cell receptor and the insulin receptor, linking activation of various signal transduction pathways with post-transcriptional gene regulation (3). SAM68 is a target for phosphorylation by Src-related kinases and Erk1/2 mitogen-activated protein kinases. Phosphorylation of SAM68 regulates both its affinity for RNA and alternative splicing of RNAs like CD44 receptor mRNA (1,4). Many of the activities of SAM68 and its targets for alternative splicing have been implicated in the development and progression of spinal muscular atrophy and cancers such as prostate and breast carcinomas, where SAM68 is frequently upregulated (1,5-8).
- Bielli, P. et al. (2011) Endocr Relat Cancer 18, R91-R102.
- Lukong, K.E. and Richard, S. (2003) Biochim Biophys Acta 1653, 73-86.
- Najib, S. et al. (2005) Cell Mol Life Sci 62, 36-43.
- Matter, N. et al. (2002) Nature 420, 691-5.
- Pedrotti, S. et al. (2010) EMBO J 29, 1235-47.
- Busà, R. et al. (2007) Oncogene 26, 4372-82.
- Rajan, P. et al. (2008) J Pathol 215, 67-77.
- Song, L. et al. (2010) J Pathol 222, 227-37.
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