Revision 3

#82470

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Orders:

877-616-CELL (2355)

orders@cellsignal.com

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P

Reactivity:
M

Sensitivity:
Endogenous

MW (kDa):
220

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q62230

Entrez-Gene Id:
20612

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:1000 - 1:4000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #94578.

Specificity/Sensitivity

Siglec-1/CD169 (E1D7G) Rabbit mAb recognizes endogenous levels of total Siglec-1/CD169 protein. This antibody does not cross-react with human Siglec-1/CD169 protein. Non-specific staining was observed in mouse pancreas and testis by immunohistochemistry.


Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1229 of mouse Siglec-1/CD169 protein.

Background

Sialic acid-binding Ig-like lectin 1 (Siglec-1/CD169) is a type-I transmembrane glycoprotein and a member of the siglec family of sialic acid-binding proteins. Structurally, Siglec-1 contains a heavily glycosylated extracellular domain and a short intracellular domain with no canonical signaling motifs (1). Expression of Siglec-1 is largely restricted to tissue-resident macrophages found in the bone marrow, liver, spleen, and lymph nodes. Research studies have shown that Siglec-1 is an adhesion molecule and binds to sialylated glycoconjugates to facilitate cell-cell and cell-matrix interactions (2-4). Siglec-1 has also been shown to facilitate the host innate immune response by binding to sialylated epitopes on the surface of viral and bacterial pathogens prior to initiating their phagocytosis (5,6). In the context of cancer, Siglec-1 has been shown to serve as a marker for subsets of proinflammatory macrophages in the tumor microenvironment that aid in the anti-tumor response by phagocytosing tumor cells and recruiting cytotoxic lymphocytes (7).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Western blot analysis of extracts from mouse bone marrow-derived macrophages, untreated (-) or treated with PNGase F (+), using Siglec-1/CD169 (E1D7G) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 1: Siglec-1/CD169 (E1D7G) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human Siglec-1/CD169 protein (hSiglec-1/CD169-Myc/DDK; +) and Myc/DDK-tagged full-length mouse Siglec-1/CD169 protein (mSiglec-1/CD169-Myc/DDK; +), using Siglec-1/CD169 (E1D7G) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 2: Siglec-1/CD169 (E1D7G) Rabbit mAb
Western blot analysis of extracts from RAW 264.7 cells, untreated (-) or treated with Lipopolysaccharides (LPS) #14011 (1 μg/mL, 24 hr; +), using Siglec-1/CD169 (E1D7G) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Induction of Siglec-1/CD169 protein by LPS is consistent with published observations.
Western Blotting Image 3: Siglec-1/CD169 (E1D7G) Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded CT26.WT syngeneic tumor and surrounding stroma using Siglec-1/CD169 (E1D7G) Rabbit mAb.
Immunohistochemistry Image 1: Siglec-1/CD169 (E1D7G) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded MC38 syngeneic tumor using Siglec-1/CD169 (E1D7G) Rabbit mAb.
Immunohistochemistry Image 2: Siglec-1/CD169 (E1D7G) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded mouse thymus using Siglec-1/CD169 (E1D7G) Rabbit mAb.
Immunohistochemistry Image 3: Siglec-1/CD169 (E1D7G) Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded mouse colon using Siglec-1/CD169 (E1D7G) Rabbit mAb.
Immunohistochemistry Image 4: Siglec-1/CD169 (E1D7G) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded mouse liver using Siglec-1/CD169 (E1D7G) Rabbit mAb.
Immunohistochemistry Image 5: Siglec-1/CD169 (E1D7G) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded mouse spleen using Siglec-1/CD169 (E1D7G) Rabbit mAb.
Immunohistochemistry Image 6: Siglec-1/CD169 (E1D7G) Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded mouse lung using Siglec-1/CD169 (E1D7G) Rabbit mAb.
Immunohistochemistry Image 7: Siglec-1/CD169 (E1D7G) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded CT26.WT syngeneic tumor using Siglec-1/CD169 (E1D7G) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 8: Siglec-1/CD169 (E1D7G) Rabbit mAb
Immunohistochemical analysis of paraffin-embedded mouse bone marrow-derived macrophages (left, positive) or C2C12 cell pellet (right, negative) using Siglec-1/CD169 (E1D7G) Rabbit mAb.
Immunohistochemistry Image 9: Siglec-1/CD169 (E1D7G) Rabbit mAb
Orders: 877-616-CELL (2355) orders@cellsignal.com Support: 877-678-TECH (8324) info@cellsignal.com Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.