Render Target: STATIC
Render Timestamp: 2024-12-20T11:44:45.916Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:59:58.585
Product last modified at: 2024-12-03T19:15:09.346Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Siglec-1/CD169 (E1D7G) Rabbit mAb #82470

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 220
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:1000 - 1:4000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #94578.

    Protocol

    Specificity / Sensitivity

    Siglec-1/CD169 (E1D7G) Rabbit mAb recognizes endogenous levels of total Siglec-1/CD169 protein. This antibody does not cross-react with human Siglec-1/CD169 protein. Non-specific staining was observed in mouse pancreas and testis by immunohistochemistry.


    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1229 of mouse Siglec-1/CD169 protein.

    Background

    Sialic acid-binding Ig-like lectin 1 (Siglec-1/CD169) is a type-I transmembrane glycoprotein and a member of the siglec family of sialic acid-binding proteins. Structurally, Siglec-1 contains a heavily glycosylated extracellular domain and a short intracellular domain with no canonical signaling motifs (1). Expression of Siglec-1 is largely restricted to tissue-resident macrophages found in the bone marrow, liver, spleen, and lymph nodes. Research studies have shown that Siglec-1 is an adhesion molecule and binds to sialylated glycoconjugates to facilitate cell-cell and cell-matrix interactions (2-4). Siglec-1 has also been shown to facilitate the host innate immune response by binding to sialylated epitopes on the surface of viral and bacterial pathogens prior to initiating their phagocytosis (5,6). In the context of cancer, Siglec-1 has been shown to serve as a marker for subsets of proinflammatory macrophages in the tumor microenvironment that aid in the anti-tumor response by phagocytosing tumor cells and recruiting cytotoxic lymphocytes (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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