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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Siglec-1/CD169 (E1D7G) Rabbit mAb #82470

Filter:
  • WB
  • IHC
Western Blotting Image 1: Siglec-1/CD169 (E1D7G) Rabbit mAb
Western blot analysis of extracts from mouse bone marrow-derived macrophages, untreated (-) or treated with PNGase F (+), using Siglec-1/CD169 (E1D7G) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 82470

Cat. # Size Qty. Price
82470T 20 µl
$169
82470S 100 µl
$402

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 220
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunohistochemistry (Paraffin) 1:1000 - 1:4000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #94578.

Protocol

Specificity / Sensitivity

Siglec-1/CD169 (E1D7G) Rabbit mAb recognizes endogenous levels of total Siglec-1/CD169 protein. This antibody does not cross-react with human Siglec-1/CD169 protein. Non-specific staining was observed in mouse pancreas and testis by immunohistochemistry.


Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1229 of mouse Siglec-1/CD169 protein.

Background

Sialic acid-binding Ig-like lectin 1 (Siglec-1/CD169) is a type-I transmembrane glycoprotein and a member of the siglec family of sialic acid-binding proteins. Structurally, Siglec-1 contains a heavily glycosylated extracellular domain and a short intracellular domain with no canonical signaling motifs (1). Expression of Siglec-1 is largely restricted to tissue-resident macrophages found in the bone marrow, liver, spleen, and lymph nodes. Research studies have shown that Siglec-1 is an adhesion molecule and binds to sialylated glycoconjugates to facilitate cell-cell and cell-matrix interactions (2-4). Siglec-1 has also been shown to facilitate the host innate immune response by binding to sialylated epitopes on the surface of viral and bacterial pathogens prior to initiating their phagocytosis (5,6). In the context of cancer, Siglec-1 has been shown to serve as a marker for subsets of proinflammatory macrophages in the tumor microenvironment that aid in the anti-tumor response by phagocytosing tumor cells and recruiting cytotoxic lymphocytes (7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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