Revision 1
#14234
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
20
Source/Isotype:
Rabbit
UniProt ID:
#O00762
Entrez-Gene Id:
11065
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Ubiquitin-conjugating enzyme 2C (UBE2C) is one of several ubiquitin conjugating enzymes participating in the E3 anaphase-promoting complex (APC/C). UBE2C is involved in the control of multiple stages of the cell cycle including inactivation of the mitotic spindle assembly checkpoint (2). UBE2C facilitates ubiquitin-dependent proteasomal degradation by initiating K11-linked ubiquitin chains on APC/C substrates (3). Research studies show that UBE2C expression is low in normal tissues, but its expression is dramatically upregulated in tumors derived from tissues such as lung, breast, and prostate (3-8). Overexpression of UBE2C in many types of solid tumors has been attributed to genomic amplification of the UBE2C locus and research studies have suggested that inhibition of UBE2C activity may have therapeutic potential (9).
Background References
- Hershko, A. (1988) J Biol Chem 263, 15237-40.
- Reddy, S.K. et al. (2007) Nature 446, 921-5.
- Wickliffe, K.E. et al. (2011) Cell 144, 769-81.
- Okamoto, Y. et al. (2003) Cancer Res 63, 4167-73.
- Berlingieri, M.T. et al. (2007) Eur J Cancer 43, 2729-35.
- Loussouarn, D. et al. (2009) Br J Cancer 101, 166-73.
- Wang, Q. et al. (2009) Cell 138, 245-56.
- Chen, Z. et al. (2011) EMBO J 30, 2405-19.
- Wagner, K.W. et al. (2004) Oncogene 23, 6621-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human M: Mouse R: Rat Mk: Monkey
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XP is a registered trademark of Cell Signaling Technology, Inc.
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Limited Uses
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