Revision 1
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YAP/TAZ Transcriptional Targets Antibody Sampler Kit
1 Kit
(8 x 20 microliters)
877-616-CELL (2355)
877-678-TECH (8324)
3 Trask Lane | Danvers | Massachusetts | 01923 | USA
For Research Use Only. Not for Use in Diagnostic Procedures.
| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| CTGF (D8Z8U) Rabbit mAb | 86641 | 20 µl | 35 kDa | Rabbit IgG |
| CYR61 (D4H5D) XP® Rabbit mAb | 14479 | 20 µl | 41 kDa | Rabbit IgG |
| IGFBP3 (D1U9C) Rabbit mAb | 25864 | 20 µl | 40 kDa | Rabbit IgG |
| Integrin β2 (D4N5Z) Rabbit mAb | 73663 | 20 µl | 95 kDa | Rabbit IgG |
| Survivin (71G4B7) Rabbit mAb | 2808 | 20 µl | 16 kDa | Rabbit IgG |
| Axl (C89E7) Rabbit mAb | 8661 | 20 µl | 138 kDa | Rabbit IgG |
| Pan-TEAD (D3F7L) Rabbit mAb | 13295 | 20 µl | 50, 53, 55, 60 kDa | Rabbit IgG |
| Lamin B2 (D8P3U) Rabbit mAb | 12255 | 20 µl | 68 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The YAP/TAZ Transcriptional Targets Antibody Sampler Kit provides an economical means of detecting proteins whose transcription is subject to regulation by the transcriptional co-activators YAP and/or TAZ. The kit provides enough antibody to perform two western blot experiments with each primary antibody.
Storage
Background
YAP and TAZ (WWTR1) are transcriptional co-activators that play a central role in the Hippo Signaling pathway that regulates cell, tissue and organ growth. Under growth conditions, YAP and TAZ are translocated to the nucleus, where they interact with DNA-binding transcription factors (e.g., Transcriptional Enhanced Activation Domain [TEAD] proteins) to regulate the expression of genes that control fundamental aspects of cell function, such as proliferation and cell survival (1). A number of genes have been experimentally confirmed as targets of transcriptional regulation by YAP and TAZ. These include the extracellular matrix proteins CTGF, CYR61, and integrin β2 (2-4), the inhibitor of apoptosis protein (IAP) survivin (5), the mechano-sensitive nuclear envelope protein Lamin B2 (6), and the oncogenic receptor tyrosine kinase Axl (7).
Background References
- Holden, J.K. and Cunningham, C.N. (2018) Cancers (Basel) 10, .
- Zhang, H. et al. (2009) J Biol Chem 284, 13355-62.
- Chan, S.W. et al. (2011) J Biol Chem 286, 7018-26.
- Chan, S.W. et al. (2011) Oncogene 30, 600-10.
- Zhang, W. et al. (2015) Cancer Res 75, 4450-7.
- Zanconato, F. et al. (2015) Nat Cell Biol 17, 1218-27.
- Cui, Z.L. et al. Int J Immunopathol Pharmacol 25, 989-1001.
Trademarks and Patents
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.