YAP/TAZ Transcriptional Targets Antibody Sampler Kit #56674
Product Information
Kit Usage Information
Protocols
- 2808: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 7074: Western Blotting
- 8661: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 12255: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
- 13295: Western Blotting, Immunoprecipitation (Magnetic)
- 14479: Western Blotting, Immunofluorescence*, Flow
- 25864: Western Blotting, Immunoprecipitation (Magnetic)
- 73663: Western Blotting
- 86641: Western Blotting, Immunofluorescence
Product Description
Specificity / Sensitivity
Each antibody in the YAP/TAZ Transcriptional Targets Antibody Sampler Kit detects endogenous levels of its target protein. Pan-TEAD (D3F7L) Rabbit mAb was shown to react with TEAD1, 2, 3 and 4 using gene-specific transfected cell extracts.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues Gly54 of human TEAD1, Val174 of human CTGF, Pro171 of human CYR61, Leu49 of human Integrin β2, Lys435 of human lamin B2, Cys60 of human survivin, and recombinant protein fragments corresponding to human Axl, and the amino terminus of human IGFBP3.
Background
YAP and TAZ (WWTR1) are transcriptional co-activators that play a central role in the Hippo Signaling pathway that regulates cell, tissue and organ growth. Under growth conditions, YAP and TAZ are translocated to the nucleus, where they interact with DNA-binding transcription factors (e.g., Transcriptional Enhanced Activation Domain [TEAD] proteins) to regulate the expression of genes that control fundamental aspects of cell function, such as proliferation and cell survival (1). A number of genes have been experimentally confirmed as targets of transcriptional regulation by YAP and TAZ. These include the extracellular matrix proteins CTGF, CYR61, and integrin β2 (2-4), the inhibitor of apoptosis protein (IAP) survivin (5), the mechano-sensitive nuclear envelope protein Lamin B2 (6), and the oncogenic receptor tyrosine kinase Axl (7).
- Holden, J.K. and Cunningham, C.N. (2018) Cancers (Basel) 10, .
- Zhang, H. et al. (2009) J Biol Chem 284, 13355-62.
- Chan, S.W. et al. (2011) J Biol Chem 286, 7018-26.
- Chan, S.W. et al. (2011) Oncogene 30, 600-10.
- Zhang, W. et al. (2015) Cancer Res 75, 4450-7.
- Zanconato, F. et al. (2015) Nat Cell Biol 17, 1218-27.
- Cui, Z.L. et al. Int J Immunopathol Pharmacol 25, 989-1001.
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