FADS1 (F1D2A) Rabbit mAb #10076
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 43 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
FADS1 (F1D2A) Rabbit mAb recognizes endogenous levels of total FADS1 protein. This antibody does not cross-react with FADS2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe100 of human FADS1 protein.
Background
Fatty acid desaturase 1 (FADS1), also known as delta-5 desaturase, is essential for the biosynthesis of highly unsaturated fatty acids (HUFAs) from linoleic acid (LA) and α-linolenic acid (ALA), both of which are polyunsaturated fatty acids (PUFAs). In addition, FADS1 converts NADH to NAD+. Studies show that FADS1 and fatty acid desaturase 2 (FADS2) thus enable NAD+ recycling for glycolysis to support cell viability when aerobic respiration is acutely suppressed (1,2). Furthermore, FADS1 and ELOVL5 expression is enhanced in mesenchymal-type gastric cancer cells, sensitizing the cancer cells to ferroptosis. On the contrary, FADS1 and ELOVL5 expression is inhibited in intestinal-type gastric cancer cells, leading to resistance to ferroptosis (3).
Limited Uses
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