Glycolysis II Antibody Sampler Kit #12866
Product Information
Kit Usage Information
Protocols
- 3810: Western Blotting, Immunoprecipitation (Magnetic)
- 3820: Western Blotting, Immunoprecipitation (Agarose)
- 7074: Western Blotting
- 8060: Western Blotting
- 9536: Western Blotting
- 12098: Western Blotting
- 13045: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence*
- 13123: Western Blotting
Product Description
Specificity / Sensitivity
Each antibody recognizes endogenous total levels of the specified target protein independent of its modified state.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with a synthetic peptides corresponding to residues surrounding Pro263 of human fructose bisphosphate aldolase A protein, human PDHK1 protein, residues surrounding Pro454 within the fructose-2,6-biphosphatase region of human PFKFB2 protein, residues surrounding Leu456 of human PFKFB3 protein, and the carboxy terminus of human PGAM1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human enolase-1 and the carboxy terminus of human enolase-2 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.
Background
Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level. Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (2). Aldolase (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde (3). Phosphoglycerate mutase (PGAM1) catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis (4). Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers (5). Pyruvate dehydrogenase kinase (PDHK) phosphorylates PDH and inactivates it, whereas dephosphorylation of PDH is carried out by pyruvate dehydrogenase phosphatase to generate the active form (6).
- Mediavilla, D. et al. (2008) J Biochem 144, 235-44.
- Atsumi, T. et al. (2005) Diabetes 54, 3349-57.
- Castaldo, G. et al. (2000) Clin Chem 46, 901-6.
- Vander Heiden, M.G. et al. (2010) Science 329, 1492-9.
- Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
- Wigfield, S.M. et al. (2008) Br J Cancer 98, 1975-84.
Limited Uses
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