Dab2 (D7O9T) Rabbit mAb #12906
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 96 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:3200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly388 of human Dab2 protein.
Background
Disabled homolog 2 (Dab2) is a mitogen responsive phosphoprotein that exerts multiple functions through association with numerous proteins. Dab2 modulates signaling pathways through interactions with proteins such as Smads and TGF-β receptors (1,2), axin (3), GRB (4), and Src (5). Dab2 also serves as a cargo-specific adaptor of clathrin-mediated endocytosis via interaction with clathrin (6), AP2 (7), NPXY-containing cargo (8-10), and myosin VI (11,12). In addition, Dab2 regulates cell adhesion by directly binding integrins (13,14). The diverse functions of Dab2 enable it to coordinate cell adhesion, cell motility, membrane trafficking, and signaling. Research studies have shown Dab2 is downregulated in a number of cancers, thereby suggesting a role as a tumor suppressor (15-17). Phosphorylation of Dab2 decreases its endocytotic function (18).
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- Mishra, S.K. et al. (2002) EMBO J 21, 4915-26.
- Morris, S.M. and Cooper, J.A. (2001) Traffic 2, 111-23.
- Keyel, P.A. et al. (2006) Mol Biol Cell 17, 4300-17.
- Maurer, M.E. and Cooper, J.A. (2006) J Cell Sci 119, 4235-46.
- Maurer, M.E. and Cooper, J.A. (2005) J Cell Sci 118, 5345-55.
- Morris, S.M. et al. (2002) Traffic 3, 331-41.
- Hasson, T. (2003) J Cell Sci 116, 3453-61.
- Huang, C.L. et al. (2006) J Cell Sci 119, 4420-30.
- Teckchandani, A. et al. (2009) J Cell Biol 186, 99-111.
- Mok, S.C. et al. (1998) Oncogene 16, 2381-7.
- Anupam, K. et al. (2006) World J Gastroenterol 12, 6041-5.
- Bagadi, S.A. et al. (2007) Breast Cancer Res Treat 104, 277-86.
- Chetrit, D. et al. (2011) J Biol Chem 286, 5392-403.
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