Gelsolin (D9W8Y) Rabbit mAb #12953
- WB
- IHC
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 83 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #57376.
Protocol
Specificity / Sensitivity
Gelsolin (D9W8Y) Rabbit mAb recognizes endogenous levels of total gelsolin protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val377 of human gelsolin protein.
Background
Gelsolin (actin-depolymerizing factor, ADF, AGEL, Brevin) is an 83 kDa protein that shares structural and functional homology to villin and adseverin/scinderin (1,2). Gelsolin plays an important role in actin filament assembly by capping and severing actin proteins in a Ca2+-dependent manner (3,4). Gelsolin is important for cellular events (e.g., membrane ruffling, chemotaxis, ciliogenesis) that require cytoskeletal remodeling (3). Accordingly, cells from gelsolin knockout mice exhibit motility defects, including a failure to ruffle in response to growth factor stimulation (5,6). In humans, defects in gelsolin have been linked to amyloidosis type 5 (AMYL5), a hereditary disease characterized by cranial neuropathy, which appears to result from gelsolin amyloid deposition (7).
- Vitale, M.L. et al. (1991) J Cell Biol 113, 1057-67.
- Lueck, A. et al. (1998) J Cell Sci 111 ( Pt 24), 3633-43.
- Sun, H.Q. et al. (1999) J Biol Chem 274, 33179-82.
- Li, G.H. et al. (2010) Med Res Rev, 32, 999-1025.
- Azuma, T. et al. (1998) EMBO J 17, 1362-70.
- Lu, M. et al. (1997) J Cell Biol 138, 1279-87.
- Haltia, M. et al. (1990) Biochem Biophys Res Commun 167, 927-32.
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