UFD1 Antibody #13789
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UFD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The ubiquitin fusion degradation 1 (UFD1) adaptor protein is a component of a protein complex essential for degradation of misfolded proteins by the endoplasmic reticulum-associated protein degradation (ERAD) pathway (1). The UFD1 protein contains a pair of conserved, amino-terminal ubiquitin-binding sites responsible for binding mono- and polyubiquitin molecules (2,3). The carboxy-terminal region of UFD1 contains binding sites for both the adapter protein NPL4 and the AAA ATPase VCP (4). The UFD1-NPL4 heterodimer binds VCP to create a protein complex responsible for export of misfolded proteins from the ER to the cytoplasm for ubiquitin-mediated degradation (5-7). The same protein complex may also be involved in disassembly of the spindle apparatus following mitosis (8).
- Ye, Y. et al. (2001) Nature 414, 652-6.
- Ye, Y. et al. (2003) J Cell Biol 162, 71-84.
- Park, S. et al. (2005) Structure 13, 995-1005.
- Bruderer, R.M. et al. (2004) J Biol Chem 279, 49609-16.
- Meyer, H.H. et al. (2000) EMBO J 19, 2181-92.
- Meyer, H.H. et al. (2002) EMBO J 21, 5645-52.
- Pye, V.E. et al. (2007) Proc Natl Acad Sci U S A 104, 467-72.
- Cao, K. and Zheng, Y. (2004) Cell Cycle 3, 422-4.
Limited Uses
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