PathScan® Total BACE Sandwich ELISA Kit #13907
Important Ordering Details
Custom Ordering Details:When ordering five or more kits, please contact us for processing time and pricing.
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Supporting Data
| REACTIVITY | H R |
Species Cross-Reactivity Key:
- H-Human
- R-Rat
Product Information
Product Description
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
PathScan® Total BACE Sandwich ELISA Kit detects endogenous and transfected levels of BACE protein as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Human, Rat
Background
β-secretase (BACE) is an aspartic acid proteinase that catalyzes the initial step in APP processing by cleaving and releasing a soluble, extracellular APP-β (sAPPβ) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99 amino acids (CTF99). Additional processing of CTF99 by γ-secretase generates the amyloid β-peptide (Aβ) that forms aggregates in the brains of Alzheimer disease (AD) patients. BACE is an attractive target for inhibitors in AD therapy since it catalyzes the first and rate limiting step in amyloidogenic APP processing (1). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (2). BACE-1 functions as a dimer and is phosphorylated at Ser498 by casein kinase 1, which is necessary for recycling back to the membrane from the early endosome (3,4).
Limited Uses
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