QKI (E7O4A) Rabbit mAb #23065
- WB
- IP
- IF
- eCLIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 38, 42 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- eCLIP-eCLIP
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Frozen) | 1:400 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
| eCLIP | 1:200 |
Storage
Protocol
Specificity / Sensitivity
QKI (E7O4A) Rabbit mAb recognizes endogenous levels of total QKI protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro11 of human QKI protein.
Background
Quaking (QKI) is a member of the signal transduction and activator of RNA (STAR) protein family of RNA-binding proteins (1,2). Mutations in the mouse Qki locus results in impaired myelin formation resulting in tremors (3). QKI proteins exist as homodimers, and disruption of the dimerization process is lethal in mice (2). QKI exists in different isoforms that differ in their C-terminus, resulting in unique subcellular localizations (4). The nuclear isoform of QKI, QKI-5, is involved in regulation of alternative splicing of MAG mRNA, which encodes for a protein important for myelin sheath formation and maintenance (4,5). QKI has also been implicated in schizophrenia and oligodendrocyte differentiation (6). QKI can function as a tumor suppressor, as it is regulated by p53 to stabilize miRNAs that regulate TGF-β signaling (7).
- Vernet, C. and Artzt, K. (1997) Trends Genet 13, 479-84.
- Chen, T. and Richard, S. (1998) Mol Cell Biol 18, 4863-71.
- SIDMAN, R.L. et al. (1964) Science 144, 309-11.
- Wu, J. et al. (1999) J Biol Chem 274, 29202-10.
- Wu, J.I. et al. (2002) Proc Natl Acad Sci U S A 99, 4233-8.
- Aberg, K. et al. (2006) Proc Natl Acad Sci U S A 103, 7482-7.
- Chen, A.J. et al. (2012) Genes Dev 26, 1459-72.
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