NR2F6/EAR-2 (E7Q7G) Rabbit mAb #24847
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 43 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Protocol
Specificity / Sensitivity
NR2F6/EAR-2 (E7Q7G) Rabbit mAb recognizes endogenous levels of total NR2F6/EAR-2 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NR2F6/EAR-2 protein.
Background
NR2F6 (Nuclear receptor subfamily 2 group F member 6, EAR-2, COUP-TFIII) is a member of the NR2F family of transcription factors, defined by their role in the regulation of organogenesis, neurogenesis, and cellular differentiation during embryonic development (1,3). NR2F6 acts as a negative regulator for TRβ1-mediated transcriptional activity, a transcriptional repressor of IL-17 expression in Th-17 differentiated CD4+ T cells, and a repressor of the NFAT/AP-1 complex on both the interleukin-2 and the interferon-γ cytokine promoters (2-5). NR2F6 has a non-redundant role in antagonizing anti-cancer effector T cell responses (5). It has been shown that loss of NR2F6 enhances the activity of established PD-L1 checkpoint blockade, promoting tumor regression and increased survival in mouse models (5,6).
- Ladias, J.A. et al. (1992) J Biol Chem 267, 15849-60.
- Zhu, X.G. et al. (2000) Mol Cell Biol 20, 2604-18.
- Hermann-Kleiter, N. et al. (2008) Immunity 29, 205-16.
- Hermann-Kleiter, N. and Baier, G. (2014) Cell Commun Signal 12, 38.
- Hermann-Kleiter, N. et al. (2015) Cell Rep 12, 2072-85.
- Klepsch, V. et al. (2018) Nat Commun 9, 1538.
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